HLA B57 Screen for Abacavir Sensitivity
HLA-B*5701 Screening for Abacavir Hypersensitivity, EPIC: LAB6425, SOFT: CIB57
Specimen Collection Criteria
Collect: One Lavender-top EDTA tube.
Invert several times to mix well.
Physician Office/Drawsite Specimen Preparation
Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.
Preparation for Courier Transport
Transport: Whole blood at room temperature (20-26°C or 68-78.8°F).
- Unlabeled specimens.
- Specimens drawn in clot, SST, or Heparin tubes.
- This test will only be performed once in a lifetime for a patient.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Frozen (-80°C): 5 years (DNA)
Royal Oak HLA Laboratory
Monday - Friday, 7:00 a.m. - 5:00 p.m.
Results available within 7 days.
Negative or positive for HLA-B*57:01.
Sequence Specific Priming (SSP) for HLA Class I Genotyping.
If HLA-B57 is identified, high resolution allele-specific typing will be performed to determine if B*5701 is present.
The FDA recommends that genetic testing for HLA-B*5701 be performed prior to prescribing the reverse transcriptase inhibitor, Abacavir, to HIV patients. The presence of HLA-B*5701 is associated with a drug-induced hypersensitivity reaction to Abacavir that can be prevented with prior genetic screening.
Human Immunodeficiency Virus (HIV).
- Mallal et al. HLA-B*5701 Screening for Hypersensitivity to Abacavir. The New England Journal of Medicine, 358 (6): 568-579, 2008.
EPIC: LAB6425, SOFT: CIB57
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.
This directory currently reflects information only for specimens collected and/or processed at the Farmington Hills,
Grosse Pointe, Royal Oak, and Troy campuses.