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Plasminogen Activator Inhibitor 1 Activity

Test Codes

EPIC: LAB6318, Beaker: XPAIA, Mayo: PAI1

Department

Send Outs

Specimen Collection Criteria

Collect: One Light Blue-top Sodium Citrate tube.

Critical frozen. Send specimen for processing immediately after collection.

Physician Office/Draw Specimen Preparation

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 4 hours of collection. If transport within 4 hours is not possible, the following procedure must be followed for accurate results:

1. Centrifuge the capped tubes at 1500 x g for 10 minutes.
2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 10 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
3. Immediately remove only the top two-thirds of the PPP and aliquot it into plastic tubes.
4. Freeze (-20°C/-4°F or below) the PPP specimens immediately.
5. Transport frozen on DRY ICE. Specimens must remain frozen during transport.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-26°C or 68-78.8°F), or 1.0 mL platelet poor plasma (PPP) obtained through the process listed above, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL PPP)

Rejection Criteria

• Clotted specimen.
• Grossly hemolyzed specimen.
• Grossly lipemic specimen.
• Grossly icteric specimen.
• Non-citrated plasma.
• Non-frozen specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Maintain platelet poor plasma (PPP) frozen (-20°C/-4°F or below) until transport. If whole blood is received, obtain PPP through the process below:

1. Centrifuge the capped tubes at 1500 x g for 10 minutes.
2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 10 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
3. Immediately remove only the top two-thirds of the PPP and aliquot it into plastic tubes.
4. Freeze (-20°C/-4°F or below) the PPP specimens immediately.
5. Transport frozen on DRY ICE. Specimens must remain frozen during transport.

Transport: 1.0 mL platelet poor plasma (PPP) obtained through the process listed above, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL PPP)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 180 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratories in Rochester, MN.

Performed

Wednesday.
Results are available in 7-12 days.

Reference Range

By report.

Test Methodology

Enzyme Linked Immunosorbent Assay (ELISA).

Interpretation

By report.

Clinical Utility

Plasminogen Activator Inhibitor I (PAI-I) is a plasma protein that rapidly and specifically inhibits tissue Plasminogen Activator. It plays a role in the development of arterial and venous thromboembolism. Elevated levels are found in patients with Systemic Lupus Erythematosis and in patients with Hypofibrolysis. Levels are correlated with insulin release. It shows an independent risk factor for myocardial infarction and hyperinsulinemia – insulin resistance. Attempts to reduce insulin resistance also reduce levels of PAI-I. Levels of PAI-I are elevated in coronary artery disease. In patients with glucose resistance, PAI-I may be the best predictor of disease progression or coronary atherosclerosis.

CPT Codes

85415

Contacts

Last Updated

9/16/2023