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Neutrophil Oxidative Burst

Test Codes

EPIC: LAB6308, Beaker: XNOB, Mayo: DHRP

Department

Send Outs

Instructions

• A control specimen from a healthy, non-related individual must be collected.
• Collect specimens Monday – Thursday, before 12:00 pm only. Specimens must be sent out the same day as collection.
• Contact the Send Outs Laboratory at 248-511-9045 prior to specimen collection with questions.

Specimen Collection Criteria

Collect: One Green-top Sodium Heparin tube from the patient and one Green-top Sodium Heparin tube as a normal control specimen from a healthy, unrelated individual in identical tubes.

• Specimens must remain in original tubes. Critical Ambient. Do not centrifuge or refrigerate.
• Send patient specimen and control specimen together.

Physician Office/Draw Specimen Preparation

Do not centrifuge, refrigerate, or freeze specimens. Maintain specimens in original collection tubes and transport STAT to the Laboratory.

Preparation for Courier Transport

Transport: 5.0 mL whole blood from the patient, at room temperature (20-26°C or 68-78.8°F) and 5.0 mL whole blood control specimen, also at room temperature (20-26°C or 68-78.8°F). (Minimum: 1.0 mL)

Rejection Criteria

• Specimens that are refrigerated or frozen.
• Specimens that are older than 48 hours before arrival at Mayo Laboratories.
• Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge, refrigerate, or freeze specimens. Maintain specimens in original collection tubes.

Transport: 5.0 mL whole blood from the patient, at room temperature (20-26°C or 68-78.8°F) and 5.0 mL whole blood control specimen, also at room temperature (20-26°C or 68-78.8°F). (Minimum: 1.0 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratories in Rochester, MN.

Performed

Monday – Friday
Results available in 4-5 days.

Reference Range

By Report.

Test Methodology

Semi-Quantitative Flow Cytometry.

Clinical Utility

• Normal neutrophil function is dependent on a number of properties including migration toward the site of infection, phagocytosis, and anti-microbial activity. Measuring the Neutrophil Oxidative Burst indirectly assesses anti-microbial activity.
• Patients with Chronic Granulomatous disease (CGD) may have an absent oxidative burst due to defects in neutrophil function.

CPT Codes

86352

Contacts

Last Updated

10/8/2023