Endomysial Antibody and Titer, IgG

Test Codes

EPIC: LAB1231361, Beaker: XENDG, WARDE: ENDG

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to plastic transport tube and freeze (-20°C/-4°F or below) prior to transport.

Preparation for Courier Transport

Transport: 2.0 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.3 mL)

Rejection Criteria

• Grossly hemolyzed specimens.
• Lipemic specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to plastic transport tube and freeze (-20°C/-4°F or below) prior to transport.

Transport: 2.0 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.2 mL) 

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Warde Medical Laboratory, Ann Arbor, MI, forward to Quest Diagnostics Nichols Institute of Valencia, Valencia, CA.

Performed

Monday – Friday.
Results available in 6-8 days.

Reference Range

Endomysial IgG: Screen Negative

Test Methodology

Immunofluorescence Assay (IFA).

Clinical Utility

Gluten-sensitive enteropathy is characterized as a non-allergic hypersensitivity to gluten or storage proteins found in wheat and other cereals. This hypersensitivity causes intestinal villous atrophy resulting in malabsorption. The Endomysial IgG Antibody assay may aid in the diagnosis of gluten-sensitivity enteropathy (i.e., celiac disease, dermatitis herpetiformis) in Endomysial IgA negative patients with confirmed IgA deficiency. A negative Endomysial IgG test alone does not rule out gluten-sensitive enteropathy. These antibodies generally decrease or become negative in patients on gluten-free diets and will usually reappear upon gluten challenge.

CPT Codes

86255, 86256 (if indicated).

Contacts

Last Updated

2/6/2026