Lab Test

Thiocyanate Level

Nitroprusside, Nipride

Test Codes

Antrim #17067, EPIC: LAB5420, SOFT: THIOC



Specimen Collection Criteria

Collect: One plain Red-top tube. (Minimum Whole Blood: 3.0 mL)

Do not use Serum Separator Tubes.

Record the exact time of specimen collection on the tube or in the computer system.

Physician Office/Draw Specimen Preparation

Let specimens clot 30-60 minutes. Refrigerate (2-8°C or 36-46°F) the collection tube within two hours of collection. (Minimum: 3.0 mL blood)

Preparation for Courier Transport

Transport: Collection tube, refrigerated (2-8°C or 36-46°F). (Minimum: 3.0 mL blood)

Rejection Criteria

  • Serum Separator (SST) tubes.
  • Severely lipemic or hemolyzed specimens.

In-Lab Processing

Let specimens clot 30-60 minutes. Centrifuge specimen to separate serum from cells. Deliver an aliquot of serum or the collection tube immediately to the appropriate testing station.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Frozen (-20°C/-4°F or below): 2 months


Royal Oak Toxicology Laboratory


Sunday – Saturday, 24 hours a day.
STAT results available within 1 hour of receipt in the Laboratory.
Routine results available within 24 hours.

Reference Range

Therapeutic range: 2-10 mg/dL.
Critical: Greater than 15 mg/dL.

Test Methodology

Spectrophotometric Method.

This test was developed and its performance characteristics determined by Beaumont Health. It has not been cleared or approved by the FDA. The Laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes and should not be regarded as investigational or for research.


Sodium nitroprusside releases cyanide ion which is converted in the body to thiocyanate. High thiocyanate serum concentrations may result from acute exposure to cyanide or chronic exposure to sodium nitroprusside. Thiocyanate toxicity may occur in patients with renal failure and long-term continuous IV infusions.

Clinical Utility

This assay is used to monitor the therapeutic drug level and evaluate the toxicity of thiocyanate (nitroprusside). Thiocyanate (nitroprusside) is a rapid-acting hypotensive agent used to treat hypertensive crisis and to produce controlled hypotension during surgery.

CPT Codes

LOINC: 3002-3


Last Updated


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