Lab Test

Thiamin, Blood

Vitamin B1, Thiamine

Test Codes



Send Outs


Do not use this test to monitor thiamine supplementation. Use this test to determine thiamine deficiency.

Specimen Collection Criteria

Collect: One Lavender- top EDTA (primary tube).

Physician Office/Draw Specimen Preparation

Protect specimen from light. Do not centrifuge.

  • Pour whole blood over into light- protected transport tube and freeze (-20°C/-4°F or below)
  • If unable to pour over, keep refrigerated and protected from light. Stable up to 6 hours.

Also acceptable: Frozen Lavender EDTA (primary tube) protected from light.

Preparation for Courier Transport

Transport: 2.0 mL light-protected whole blood, refrigerated (2-8°/36-46°F or below). (Minimum: 1.0 mL).

  • For pour over or frozen lavender EDTA (primary tube) specimens keep frozen (-20°C/-4°F or below) and protected from light.

Rejection Criteria

  • Clotted specimens.
  • Centrifuged specimens.
  • Specimens not light-protected.
  • Specimens not collected and processed as indicated.

In-Lab Processing

Protect specimen from light. Do not centrifuge. Pour whole blood over into light-protected transport tube.

Frozen Lavender (EDTA) specimen (primary tube) acceptable to send to reference lab, only if protect from light.

Transport: 2.0 mL light-protected whole blood, frozen (-20°C/-4°F or below). (Minimum: 1.0 mL).


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 6 hours
Frozen (-20°C/-4°F or below): 2 months

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.


Sent to Warde Medical Laboratory, Ann Arbor, MI.


Monday – Friday.
Results available in 3-6 days.

Reference Range

By report.

Test Methodology

Quantitative High Performance Liquid Chromatography (HPLC).

CPT Codes

LOINC:  2998-3


Last Updated


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