Prograf, FK506, Tacrolimus/FK506 Level
Antrim #30730, EPIC: LAB5418, SOFT: TACRO
Specimen Collection Criteria
Collect: One Lavender-top EDTA tube. (Minimum Whole Blood: 2.0 mL)
Do not use Serum Separator Tubes.
Time of Collection: Trough, just prior to next dose.
Physician Office/Draw Specimen Preparation
Do not centrifuge. Maintain whole blood in original collection tube at room temperature (20-26°C or 68-78.8°F) prior to transport.
Preparation for Courier Transport
Transport: Whole blood in original collection tube at room temperature (20-26°C or 68-78.8°F). (Minimum: 2.0 mL)
- Serum Separator (SST) tubes.
- Serum or plasma specimens.
Do not centrifuge specimen. Deliver immediately to the appropriate testing station.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 3 days
Frozen (-20°C/-4°F or below): 2 weeks
Royal Oak Toxicology Laboratory
Sunday – Saturday. Specimens must be received prior to 1:00 pm to be tested the same day.
Therapeutic range (0-3 months post transplant): 8-16 ng/mL.
Therapeutic range (Greater than 3 months post transplant): 5-15 ng/mL.
Critical: Greater than 20 ng/mL.
Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS).
This test was developed and its performance characteristics determined by Beaumont Health. It has not been cleared or approved by the FDA. The Laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes and should not be regarded as investigational or for research.
The following drugs may increase Tacrolimus blood levels: ketoconazole, fluconazole, erythromycin, diltiazem, cimetidine, and methylprednisolone.
The following drugs may decrease Tacrolimus blood levels: carbamazepine, phenobarbital, phenytoin, rifabutin, and rifampin.
Tacrolimus is an immunosuppressant drug that has been approved by the FDA for use in liver transplant patients. This assay is used to monitor the therapeutic drug level and evaluate the toxicity of Tacrolimus/ FK506. This assay aids in monitoring changes in blood concentrations resulting from interactions with coadministered drugs. Nephrotoxicity and neurotoxicity are the most significant side effects of tacrolimus.
Toxicology Laboratory – RO
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