Specimen Collection Manual: Urine for Urinalysis
- Collect at least 20 mL of clean-catch urine in a sterile collection-transfer container. Replace the blue cap on the container. Note: This container is used ONLY for collection and transfer of the urine specimen. It should NOT be used to transport specimen to the Laboratory because these containers often leak.
- The patient should cleanse themself with the towelettes as follows:
- Wipe the head of the penis in a single motion with the first towelette. Repeat with the second towelette. Uncircumcised patients should hold back the foreskin throughout the whole cleansing and urine collection process.
- Urinate a small amount into the toilet. This flushes most contaminants from the urethra.
- After a single stream is achieved, pass the specimen cup into the stream and obtain urine sample.
- Sit comfortably on the toilet and swing one knee to the side as far as possible.
- With the forefingers of one hand, spread the outer folders of the labia and keep them apart during the rest of the collection procedure.
- With the other hand, wipe the inner labial folds front to back in a single motion with the first towelette.
- Wipe down through the center of the labial folds with the second towelette.
- Keep the labia separated and urinate a small amount into the toilet. This flushes most contaminants from the urethra.
- After a single stream is achieved, pass the specimen cup into the stream and obtain sample.
- The cup should be at least 1/4 to 1/2 full.
- To prevent contamination of specimen with skin flora, the patient should remove the cup before the flow of urine stops and before releasing the labia or penis.
- The patient should then completely empty their bladder of urine into the toilet.
- Wipe off the outside of the container after replacing the cap.
- The patient must wash their hands.
- Label the specimen and deliver to the Laboratory within 30 minutes of collection. If delivery is not possible within 30 minutes, the specimen must be refrigerated.
Physician Office/Draw Specimen Preparation
- Peel back the yellow protective label to reveal the collection needle on top of the sterile urine container's blue screw cap.
- Invert the 8 mL Yellow-top conical Urinalysis evacuated tube, shown below, onto the collection needle. Firmly press the tube onto the collection needle.
- The tube will fill with urine. When the tube is full, or 3.0 mL minimum required is transferred, remove the tube from the collection needle.
- Replace the yellow protective label over the collection needle on the container's blue screw cap.
- Properly label the evacuated Yellow-top conical tube with an LIS collection label for Urinalysis.
- Send the Yellow-top conical tube to Urinalysis Laboratory.
- If a Urine Culture specimen is requested with Urinalysis, fill a Gray-top Urine Culture evacuated tube shown below (contains lyophilized urine maintenance formula) following the instructions given above in steps 3 and 4. Invert the tube several times to mix.
- Clinical Pathology requires SEPARATE specimens for Urine Culture and Urinalysis.
- Should urine volume be insufficient (less than 3.0 mL) to fill the recommended Yellow-top evacuated tube for Urinalysis (8 mL) and Gray-top tube for Culture (3 mL), see acceptable MINIMUM requirement below:
||Yellow-top tube (or Orange sterile screw-top container, tightly capped)
||Orange sterile screw-top container, tightly capped (shown below)
- Test requests for Urinalysis will be cancelled as "Quantity Not Sufficient (QNS)" for specimen volumes less than 1.0 mL received.
- It is imperative that unpreserved urine be delivered promptly to the Lab.
- Properly discard any remaining urine. Dispose of the blue screw-cap container into an appropriate sharps container.
Urinalysis Laboratory – RO
Hematology/Coagulation Laboratory – TR
Hematology/Urinalysis Laboratory – GP
Hematology/Coagulation Laboratory – FH
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