Specimen Collection Manual: Body Fluids for Culture
Specimen Collection Criteria
- Collect 3-5 mL of pleural fluid, pericardial fluid, or other body fluid in a sterile collection container.
- Most body fluids contain enough protein to stabilize viruses, bacteria, and fungi. Do not add bacterial or viral transport media to the specimen.
- Specimens for Bacterial or Fungal Culture should be transported at room temperature (20-26°C or 68-78.8°F).
- Specimens for viral detection should be sent to the Laboratory on wet ice. Do not freeze. Specimens may be stored for up to 4 hours at room temperature (20-26°C or 68-78.8°F). If longer delays are anticipated, specimens should be frozen at -70°C or below. Refrigeration is critical for body fluids because the viruses isolated from these sites are often extremely labile.
Microbiology Laboratory – RO
Microbiology Laboratory – TR
Microbiology Laboratory – GP
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