Solid Tumor/Tissue Karyotype
Karyotyping, Chromosome Analysis, Karyogram, genetic, GSTGT, cytogenetics
Specimen Collection Criteria
Collect: 1.0 x 1.0 cm piece of tumor tissue or lymph node. Place tissue in a sterile centrifuge tube containing tissue culture medium.
- Obtain specimens under sterile conditions.
- If possible, all specimens should be received in the Cytogenetics Laboratory within 3 hours of resection.
- A copy of the requisition must be sent with the specimen. The specimen and requisition form should be placed in a sealed transport bag. The requisition form should contain the patient's name, birth date, medical record number, requesting physician, specimen type and test request, date and time of collection, and tentative diagnosis.
- Additionally, a request for Cryopreservation needs to be written on the requisition form, if required.
- Fixed or frozen tissue.
- Specimens arriving contaminated or suboptimal in size.
- Specimen should be received in the Laboratory within 48 hours of collection.
- Specimen will be rejected if received in the laboratory after 72 hours of collection.
Specimens are processed immediately upon receipt in the Laboratory. Store tissue in tissue culture medium.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 48 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days. A backup culture (solid tumor in-situ) is maintained for two weeks past sign-out of the final report. A backup cell pellet (lymphoma suspension) is maintained for three weeks past sign-out of the final report.
Royal Oak Cytogenetics Laboratory
Monday – Friday, 8:00 am – 5:00 pm.
Results available within 12-14 days of receipt in the Laboratory.
Normal results would be 46,XX or 46,XY with no apparent abnormalities present.
High-resolution microscopic analysis of chromosomes to provide a digital image karyotype. Special stains or techniques such as C-Banding, NOR Banding and FISH may be required to further identify specific chromosomal abnormalities or variants.
Based on completion of chromosomal analysis. Interpretation will be issued in the form of a final report.
Karyotyping (chromosome analysis) is performed on solid tissue tumors and lymph nodes to determine the presence of chromosomal abnormalities.
Implication for clinical disease will be clearly stated in the final report.
88239, 88262, 88280, 88285.
Cytogenetics Laboratory – RO
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.