Pregnenolone, LC/MS

Test Codes

EPIC: LAB1232004, Beaker: Pregnenolone, Quest: 31493

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Allow blood to clot (30 minutes) at room temperature, 18-25°C. Centrifuge and separate the serum from the cells. Freeze as soon as possible.

Preparation for Courier Transport

Transport: 0.5 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.2 mL)

Rejection Criteria

• Gross hemolysis.
• Serum separator tube (SST).
• Specimens not collected and processed as indicated.

In-Lab Processing

Allow blood to clot (30 minutes) at room temperature, 18-25°C. Centrifuge and separate the serum from the cells. Freeze as soon as possible.

Transport: 0.5 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.2 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 72 hours
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Sunday – Friday.
Results are available in 3-6 days.

Reference Range

Adult	22-237 ng/dL
Pediatric
1-59 days	68-1303 ng/dL
60 days-1 year	≤219 ng/dL
2-6 years	≤140 ng/dL
7-9 years	≤156 ng/dL
10-12 years	15-220  ng/dL
13-17 years	12-196 ng/d

Test Methodology

Chromatography / Mass Spectrometry

CPT Codes

84140

Contacts

Last Updated

12/17/2025