PML-RARA T(15;17), Quantitative, Real Time PCR

Acute promyelocytic leukemia (APL), acute myeloid leukemia (AML), Translocation (15;17), PML/RARA, Quantitative RT-PCR, AML (M3), APL, Acute Promyelocytic Leukemia, PML/RARA Quantitative, PCR

Test Codes

EPIC: LAB1232093, Beaker: PML/RARA PCR, Quest: 14994

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): 15.0 mL blood or 43.0 mL bone marrow in one Lavender-top EDTA tube. (Minimum: 3.0 mL blood, 1.0 mL bone marrow).

Also acceptable: Whole blood or bone marrow collected in: ACD-B (yellow-top) tube or sodium heparin (green-top) tube 50.0 uL of 10.0 ng/uL extracted RNA submitted in a microcentrifuge tube. (Only accept extracted RNA when extraction or isolation is performed in an appropriately qualified laboratory such as a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by the CAP and/or CMS). (Minimum: 20.0 uL extracted RNA)

Physician Office/Draw Specimen Preparation

Do not centrifuge. Maintain specimen in original collection tube, at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: 5.0 mL blood or 3.0 mL bone marrow, at room temperature (20-26°C or 68-78.8°F) in original collection tube. (Minimum: 3.0 mL blood, 1.0 mL bone marrow).

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge. Maintain specimen in original collection tube, at room temperature (20-26°C or 68-78.8°F).

Transport: 5.0 mL blood or 3.0 mL bone marrow, refrigerated (2-8°C or 36-46°F) in original collection tube. (Minimum: 8.0 mL blood, 1.0 mL bone marrow).

Storage

Specimen Stability for Testing:

Whole Blood & Bone Marrow
Room temperature (20-26°C or 68-78.8°F): 5 days
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): Unacceptable

Extracted RNA
Room temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Preferred
Frozen (-20°C/-4°F or below): Acceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Daily.
Results available in 5-7 days.

Reference Range

By report.

Test Methodology

Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).

Interpretation

By report.

CPT Codes

81315

Contacts

Last Updated

12/17/2025