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Verify Now P2Y12

Verify Now P2Y12 assay for Antiplatelet Effect, Verify P2Y12

Test Codes

EPIC: LAB3326

Department

Coagulation

Instructions

This test is available only to inpatients drawn in Royal Oak Hospital.

Or

Outpatient Location at North MOB 3535 W 13 Mile Road Rd, Suite 147

Specialized collection kit used, notify Royal Oak STAT Lab or Royal Oak Inpatient Phlebotomy if needed for inpatient collection.

Specimen Collection Criteria

Collect: Two Greiner Light Blue-top 3.2% Sodium Citrate tubes.

Direct Venipuncture

• GREINER TUBES MUST BE FILLED TO THE BLACK LINE (1/2 tube).
• Venipuncture should be performed using a 21G or larger needle (20G or 19G). Use of a butterfly 21G is acceptable.
• If drawing blood for a CBC at the same time, fill the CBC last.
• First collect a clearing tube (at least 2.0 mL) making sure the clearing tube does not contain any platelet inhibiting substances (e.g. EDTA).
• Clearing tube does not need to be sent to the lab.

• Fill the second and third tube (Greiner sample tubes) to the black line (1/2 tube). DO NOT UNDER FILL.
• Gently invert the Griner tubes at least 5 times to ensure complete mixing of the contents.
• Keep in collection kit, both tubes must be HAND-DELIVERED to the Royal Oak STAT Lab immediately. Do NOT send through the pneumatic tube system.

Indwelling Catheter

• Discard the first 5.0 mL from an indwelling catheter to clear the line. Ensure the catheter is free of clots.
• Immediately transfer blood to both 2.0 mL Greiner sample tubes to fill volume to the black indicator line (1/2 tube). DO NOT UNDERFILL.
• If drawing blood for a CBC at the same time, fill the CBC tube last.
• Gently invert the Greiner tubes at least 5 times to ensure complete mixing of the contents.
• Keep both Greiner sample tubes in the collection kit. HAND-DELIVER to Royal Oak STAT Lab immediately.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-26°C or 68-78.8°F).

Rejection Criteria

• Specimens collected in BD light blue top 3.2% Sodium Citrate tubes.
• Specimens not collected in Greiner partial-fill vacuette tube (1/2 fill indicator) with 3.2% Sodium Citrate Blue Top.
• Specimens collected at sites other than Royal Oak Hospital campus.
• Collected specimen sent through pneumatic tube station.
• Glass tubes and 3.8% Sodium Citrate tubes.
• Specimens that are clotted or grossly hemolyzed.
• Inappropriate specimen volume, underfill or overfilled of indicator line.
• Specimens received in the Laboratory more than 4 hours past the time of collection.
• Specimen transported not in room-temperature.
• Unlabeled or mislabeled specimens Greiner Top Sample specimens.

Inpatient Specimen Preparation

Hand-deliver specimens to the Royal Oak STAT Lab immediately after collection. DO NOT use the pneumatic tube system.

In-Lab Processing

Do not refrigerate or freeze specimens. Do not centrifuge. Specimens must be analyzed within 4 hours of collection.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 4 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Room Temperature (20-26 C or 68-78.8 F): 5 days
Refrigerated (2-8°C or 36-46°F): 5 days

Laboratory

Royal Oak STAT Laboratory

Performed

Sunday – Saturday, 24 hours a day.
Results available within 4 hours of receipt in the Laboratory.

Reference Range

Verify Now P2Y12 assay results are reported in Platelet Reactivity Units (PRU).

Verify Now P2Y12 assay for effect of clopidogrel (Plavix) and related drugs on platelet activity.

Lower PRU values are indicative of effective antiplatelet drug inhibition of platelet activity.

Higher PRU values are indicative of ineffective antiplatelet drug inhibition of platelet activity.

Patients on chronic clopidogrel (Plavix) therapy with PRU values at or below 180 are considered adequate responders.

Test Methodology

Point of Care, utilizing platelet induced aggregation turbimetric optical detection.

Interpretation

See Reference Range.

Limitations:
This is a test for P2Y12 inhibition by clopidogrel (Plavix), prasugel (Effient), or ticlopidine (Ticlid). Direct IIb/IIIa inhibitors will interfere. In addition, the P2Y12 test must not be performed within 48 hours after administration of eptifibatide (Integrilin) or tirofibrin (Aggrastat), or within 14 days after administration of abciximab (ReoPro). 

Clinical Utility

The Verify Now PRUTest is a whole blood test used to measure the level of platelet P2Y12 receptor blockade. Substances known to specifically block the P2Y12 receptor include the thienopyridine class of drugs, including clopidogrel.

CPT Codes

85576

LOINC: 49010-2

Contacts

Last Updated

8/6/2025