Osteocalcin, N-MID

Bone G1a Protein

Test Codes

EPIC: LAB1232075, Beaker: Osteocalcin, Quest: 16322

Department

Send Outs

Instructions

Dietary supplements containing biotin may interfere in assays and may skew results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation.

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Allow blood to clot at room temperature (20-26°C) and centrifuge immediately to separate the serum from the cells. Freeze as soon as possible.

Preparation for Courier Transport

Transport: 1.0 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL)

Rejection Criteria

• Hemolyzed specimens.
• Heat inactivated specimen.
• Specimens stabilized with azide.
• Patient administered with biotin within 8 hours.
• Specimens not collected and processed as indicated.

In-Lab Processing

Allow blood to clot at room temperature (20-26° C) and centrifuge immediately to separate the serum from the cells. Freeze as soon as possible.

Transport: 1.0 mL serum or plasma, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 24 hours
Frozen (-20°C/-4°F or below): 21 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Monday – Saturday.
Results available in 2-4 days.

Reference Range

	Male (ng/mL)	Female (ng/mL)
0-4 Years	Not established	Not established
5-9 Years	47-142	47-142
10-13 Years	49-167	49-167
14-17 Years	26-203	14-85
>17 Years	9-38	8-3

Test Methodology

Electrochemiluminescence Immunoassay (ECLIA)

Interpretation

By report.

Clinical Utility

Osteocalcin, N-MID – Osteocalcin, the most abundant non-collagen protein in bone matrix, is a bone-specific, calcium binding protein. Serum osteocalcin levels are related to the rate of bone turnover in various disorders of bone metabolism, e.g., osteoporosis, primary and secondary hyperparathyroidism, and Paget's disease.

CPT Codes

83937

Contacts

Last Updated

12/17/2025