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Lymphocyte Antigen and Mitogen Proliferation

Test Codes

EPIC: LAB6627, Beaker: XLAMP, ARUP: 96056

Department

Send Outs

Instructions

• This test is subject to pathology review prior to specimen collection. Please call 248-551-9045 to initiate the review.
• A control specimen from a healthy, non-related individual must be collected.
• Collect specimens Monday – Thursday, before 12:00 pm only. Specimens must be sent out the same day as collection.

Specimen Collection Criteria

Collect: 10 mL whole blood in Yellow-top ACD (Solution A) or Dark Green-top Sodium Heparin tubes from the patient and 10 mL whole blood as a normal control specimen from a healthy, unrelated individual in identical tubes. (Minimum: 7 mL each from patient and control)

• Specimen must remain in original tubes. Critical Ambient. Do not centrifuge or refrigerate.
• Send patient specimen and control specimen together.

Physician Office/Draw Specimen Preparation

Do not centrifuge, refrigerate, or freeze specimens. Maintain specimens in original collection tubes and transport immediately to the Laboratory.

Preparation for Courier Transport

Transport: 10 mL whole blood, at room temperature (20-26°C or 68-78.8°F) and 10 mL whole blood control specimen from a healthy, unrelated individual, also at room temperature (20-26°C or 68-78.8°F). (Minimum: 7 mL each from patient and control)

Rejection Criteria

• Specimens in Yellow-top ACD Solution B tubes.
• Specimens that are refrigerated or frozen.
• Specimens that are older than 48 hours before arrival at ARUP Laboratories.
• Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge, refrigerate, or freeze specimens. Maintain specimens in original collection tubes and transport immediately to the Laboratory.

Transport: 10 mL whole blood, at room temperature (20-26°C or 68-78.8°F) and 10 mL whole blood control specimen from a healthy, unrelated individual, also at room temperature (20-26°C or 68-78.8°F). (Minimum: 7 mL each from patient and control) 

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to ARUP Laboratories, Salt Lake City, UT.

Performed

Tuesday – Friday.
Results available in 10-12 days.

Reference Range

By report.

Test Methodology

Cell Culture.

Clinical Utility

Lymphocyte proliferation is tested in response to a variety of antigens and mitogens to evaluate T-cell mediated immune response. Please contact Send Outs lab directly at 248-551-9045, when ordering this test.

Phytohemagglutinin, concanavalin A, pokeweed mitogen, candida antigen and tetanus antigen are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these mitogens and antigens is determined by H-thymidine incorporation. Results are reported as the counts per minute (CPM) mitogen or antigen stimulated versus a control culture and a Stimulation Index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated lymphocytes.

Clinical Disease

Patients with T-cell immunodeficiency diseases, including severe combined immunodeficiency, DiGeorge syndrome, Wiskott-Aldrich syndrome, ataxia telangiectasia, and chronic mucocutaneous candidiasis may have defective lymphocyte responses to nonspecific mitogens and/or to specific antigens, such as Candida or tetanus. Patients with severe T-cell defects, such as severe combined immunodeficiency, may be appropriate candidates for bone marrow or stem cell transplants.

CPT Codes

86353 x 5.

Contacts

Last Updated

10/10/2023