Leptospira Antibody, IgM by Dot Blot

Test Codes

EPIC: LAB1232145, Beaker: LEPTOSPIRA, Quest: 90953

Department

Send Outs

Instructions

Acute and convalescent samples must be labeled as such; parallel testing is preferred, and convalescent samples must be received within 30 days from receipt of the acute samples. Please mark sample plainly as "acute" or "convalescent."

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.2 mL)

Rejection Criteria

• Grossly lipemic specimens.
• Hemolyzed specimens.
• Any other body fluid.
• Contaminated.
• Heat-inactivated.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.2 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 1 year

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Monday, Thursday.
Results available in 2-6 days.

Reference Range

By report.

Test Methodology

Qualitative Immunoblot.

Interpretation

By report.

Clinical Utility

This assay aids in the diagnosis of leptospirosis.

CPT Codes

86720

Contacts

Last Updated

12/17/2025