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Leishmania Total Antibodies

Leishmania IgG Antibodies

Test Codes

Order as a Miscellaneous Send Out (XMISC), Mayo: LEIS

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 0.2 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.1 mL)

Rejection Criteria

• Specimens not collected and processed as indicated.
• Grossly lipemic specimen.
• Grossly hemolyzed specimen

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 0.2 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.1 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratory, Rochester, MN.

Performed

Wednesday.
Results available in 3-8 days.

Reference Range

See report.

Test Methodology

Immunochromatographic Strip Assay

Interpretation

IgG antibodies are typically detected two weeks post-infection. Titers greater than or equal to 1:32 are strongly suggestive of active disease; a titer of 1:16 in an acute specimen suggests early infection and should be followed up with a convalescent sample drawn two to three weeks later, if clinically indicated.

Differentiation of Leishmania species is not possible by this technique due to the high inter-species cross reactivity.

The clinical picture of the patient must be taken into consideration in making the final diagnosis.

Clinical Utility

This assay aids in the diagnosis of leishmaniasis.

CPT Codes

86717

Contacts

Last Updated

4/20/2023