IgG, IgA, Indirect Immunofluorescence

Human Split Skin, Monkey Esophagus, Pemphigoid, Pemphigus, Split-Skin Assay, Cutaneous Immunofluorescence Antibodies (IgG), Serum

Test Codes

EPIC: LAB1232229, Beaker: IgG IgA Incl, Quest: 16690

Department

Send Outs

Specimen Collection Criteria

Collect: One plain Red-top tube.

Do not use Serum Separator Tubes (SST).

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 6.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 3.0 mL)

Rejection Criteria

• Received frozen.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 6.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 3.0 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Monday – Friday.
Results available in 2-4 days.

Reference Range

By report.

Test Methodology

Immunoassay (IA)

CPT Codes

88346, 88350.

Contacts

Last Updated

12/17/2025