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HLA Class II Disease Association

HLA Class II Typing for Disease Association, HLA typing, tissue typing, HLA-DR, DQ typing

Test Codes

EPIC: LAB6424, SOFT: CIIDA

Department

HLA

Specimen Collection Criteria

Collect: One Yellow-top ACD tube OR one Lavender-top EDTA tube.

Invert several times to mix well.

Physician Office/Draw Specimen Preparation

Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F).

Rejection Criteria

Specimens drawn in green, clot, or SST tubes are unacceptable and will not be tested. Unlabeled specimens will be rejected.

In-Lab Processing

Maintain specimen at room temperature (20-26°C or 68-78.8°F) until testing.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Frozen (-80°C): 5 years (DNA)

Laboratory

Royal Oak HLA Laboratory

Performed

As needed.
Results typically available within 5 business days.

Test Methodology

Polymerase Chain Reaction (PCR).

Interpretation

By report.

Clinical Utility

HLA Class II genotyping defines the antigens of the HLA-DRB1 and DQB1 loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-DRB1 and DQB1 alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis for disease association purposes.

CPT Codes

81382, 81376.

Contacts

Last Updated

4/8/2024