HLA Class I Typing
HLA Class I Typing, HLA typing, HLA-A, B, C typing, tissue typing
EPIC: LAB6421, SOFT: CITYP
Specimen Collection Criteria
Collect: Two Lavender-top EDTA tubes OR two Yellow-top ACD tubes.
Invert several times to mix well.
Physician Office/Draw Specimen Preparation
Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 24 hours of collection.
Preparation for Courier Transport
Transport: Whole blood at room temperature (20-25°C or 68-77°F).
- Unlabeled specimens.
- Specimens drawn in clot, SST, or Sodium Heparin tubes.
Maintain specimen at room temperature (20-26°C or 68-78.8°F) until testing.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Frozen (-80°C): 5 years (DNA)
Royal Oak HLA Laboratory
Results typically available within 5 business days.
Polymerase Chain Reaction (PCR).
HLA Class I genotyping defines the antigens of the HLA-A, B and C loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-A, B and C alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.
HLA Laboratory - RO
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