HLA B27 Screen
HLA-Antigen B27, HLA-B27, HLA-B27 Screening Assay, Flow Cytometry, HLA B27 Screen
FB27G, EPIC: LAB6411
Specimen Collection Criteria
Collect: One Lavender-top EDTA tube.
Also acceptable: One Yellow-top ACD tube or Green-top Sodium Heparin tube.
Physician Office/Draw Specimen Preparation
Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Specimens must be received in the Laboratory within 48 hours of collection.
Preparation for Courier Transport
Transport: Whole blood, at room temperature (20-25°C or 68-77°F).
FedEx Shipping Instructions:
Transport 4 mL whole blood at room temperature. Specimen must be received within 48 hours of collection.
- Specimens received greater than 48 hours post collection are acceptable with approval of the Flow Cytometry Laboratory manager or pathologist on service.
- This test will only be performed once in a lifetime for a patient.
Maintain at room temperature (20-26°C or 68-78.8°F) prior to testing.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 48 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Room Temperature (20-26°C or 68-78.8°F): 7 days
Royal Oak Flow Cytometry Laboratory
Monday, Wednesday, Friday.
Results available in 1-3 days.
Patients will be reported as negative or positive for HLA-B27 Antigen.
Immunophenotyping by Multiparameter Flow Cytometry
This test is reported as positive or negative. If the result is in the equivocal range, the HLA test “HLA-B27 Genotyping (Spondyloarthropathies)” will be added on as a confirmatory test. A separate report will be issued. If the HLA laboratory is unable to confirm the result, the specimen will be reported as equivocal and a comment will be added: “Unable to confirm by HLA Laboratory molecular SPP testing. Recommend specimen redraw.”
Flow cytometric lymphocyte analysis has allowed for rapid and sensitive screening for the presence of HLA-B27, which may be valuable in the evaluation of patients with arthritic symptoms, as seen in ankylosing spondylitis. HLA-B27 positivity has been found to be highly associated with ankylosing spondylitis. Testing for the presence of the HLA-B27 antigen in a patient is used to assist in the diagnosis of ankylosing spondylitis and /or determine a genetic predisposition for ankylosing spondylitis.
Flow Cytometry Laboratory – RO
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