Lab Test

HIV Phenotyping

Antivirogram™, HIV Phenotype Resistance Testing

Test Codes

MONOGRAM #V3200, Testing requires pathology approval prior to collection.


Send Outs


This test is not included in the Beaumont Laboratory testing formulary, however, the Laboratory can facilitate specimen collection.

  • This test requires pathology review. Contact the Send Outs Laboratory at 248-551-9045 before ordering this test.
  • Testing requires the ordering physician to complete forms for the independent clinical laboratory performing the testing.
  • This test will be billed to the patient's insurance. If not covered by insurance, the patient is responsible for the full cost of testing.
  • Specimens received without the appropriate forms and information will not be shipped.
  • Once approved, order test as a Miscellaneous Send Out (XMISC). 

Specimen Collection Criteria

Collect: Three Lavender-top EDTA tubes, after obtaining necessary pathology approval.

Physician Office/Draw Specimen Preparation

Centrifuge to separate plasma from cells within six hours of collection. Transfer plasma to a plastic transport tube and freeze (-20°C/-4°F or below) immediately.

Preparation for Courier Transport

Transport: 4.0 mL plasma, frozen (-20°C/-4°F or below). (Minimum: 3.0 mL)

Rejection Criteria

  • Specimens received without prior Laboratory notification and review.
  • Hemolyzed specimens.
  • Specimens not collected and processed as indicated.

In-Lab Processing

Centrifuge to separate plasma from cells within six hours of collection. Transfer plasma to a plastic transport tube and freeze (-20°C/-4°F or below) immediately.

Transport: 4.0 mL plasma, frozen (-20°C/-4°F or below). (Minimum: 3.0 mL)


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.


Sent to Monogram Biosciences, San Francisco, CA.


Monday – Saturday.
Results available within one month.

Reference Range

By report.

Test Methodology

Polymerase Chain Reaction (PCR) Amplification and Viral Culture.


  • Phenotyping is the actual measurement of viral resistance found by growing the virus derived from each patient in the presence of antiviral drugs.
  • Limitations: This procedure may not be successful when the viral load is less than 500 copies per milliliter of plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load.

Clinical Utility

This assay detects resistance in HIV-1 to antiretroviral drugs.


  1. Roos MTL, Lange JMA, deGoede REY. Viral phenotype and immune response in primary human immunodeficiency virus type 1 infection. J Infect Dis 1992; 165:427-32.

CPT Codes

87903, 87904x11.


Last Updated


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