Lab Test

HIV 1/2 (RAPID)/Hepatitis Source Patient Panel

Source Patient Panel, HIV Source Testing, Source HIV, RAPID HIV, Source Hepatitis, Human Immunodeficiency Virus, Hepatitis C Antibody, Anti Hepatitis C, Anti-Hepatitis C, HBsAg, Hepatitis B Surface Antigen, HIV 1/2 (RAPID) / Hepatitis Source Patient Panel

Test Codes

EPIC: LAB6648, Beaker: HSPP




  • This panel is designed ONLY for blood/body fluid exposure cases. It should NOT be used to obtain RAPID HIV results in any other clinical cases.
  • This panel includes: HIV 1/2 Antibody and HIV-1 p24 Antigen (RAPID), Hepatitis B Surface Antigen, and Hepatitis C Virus Antibody.
    1. Order the HIV (RAPID)/Hepatitis Source Patient Panel STAT on the source patient
    2. RAPID HIV tests can only be ordered for exposures (the source of exposure is to be tested, NOT the exposed person)
    3. Form 553 (Employee Illness/Accident Form) must be filled out for the exposed person.
    4. The exposed person should proceed to Occupational Health Services or the Emergency Department at the nearest Beaumont Hospital immediately for evaluation and follow-up.

Specimen Collection Criteria

Collect: 1 EDTA whole blood tube and 1 SST. (Minimum Whole Blood: 5.0 mL)

Please collect specimens from the source of the exposure. Do not send specimens collected from the exposed person.

Physician Office/Draw Specimen Preparation

Maintain whole blood refrigerated (2–8°C or 36–46°F), or at room temperature (20–26°C or 68–78.8°F) prior to transport. For delays in transport greater than 24 hours, centrifuge the specimen, transfer plasma to a plastic transport tube, and freeze (-20°C/-4°F or below). (Minimum: 1.0 mL plasma)

Preparation for Courier Transport

Transport: Whole blood, refrigerated (2–8°C or 36–46°F), or plasma, frozen (-20°C/-4°F or below). (Minimum: 3.0 mL whole blood or 1.0 mL plasma)

Rejection Criteria

  • Heat inactivated serum.
  • Severely lipemic or hemolyzed specimens.
  • Specimens that have obvious bacterial contamination.

In-Lab Processing

Deliver immediately to the appropriate testing station.


Specimen Stability for Testing:

Whole Blood in EDTA
Room Temperature (20-26°C or 68-78.8°F): 2-4 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

EDTA Plasma aliquots
Room Temperature (20-25°C or 68-77°F): 2-4 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): For longer storage.


Dearborn Chemistry Laboratory
Farmington Hills Chemistry Laboratory
Grosse Pointe Chemistry Laboratory
Royal Oak Automated Chemistry Laboratory
Troy Chemistry Laboratory


Sunday – Saturday, 24 hours a day.
HIV 1/2 (RAPID): STAT results available within 2 hours.
HBsAg and HCV Antibody: Results available within 24 hours. 
HCV RNA Quantitation: Performed once per week, results available on Wednesday.

Reference Range

HIV 1/2 (RAPID): Non-reactive.
HBsAg: Non-reactive.
HCV Antibody: Non-reactive. 

Test Methodology

HIV (RAPID): Chemiluminescent immunoassay
HBsAg: Enzyme Chemiluminescent immunoassay with Neutralization.
HCV Antibody:  Chemiluminescent immunoassay 


A positive RAPID HIV test is considered to be a preliminary positive. HIV-1 or HIV-2 antibodies will be confirmed by the BioRad Geenius assay. When necessary additional confirmation procedures will be performed. In accordance with recent CDC guidelines, Western Blot will NOT be performed. 

Clinical Utility

  • The RAPID HIV-1/2 test is used to assay serum from source patients who have inadvertently exposed health care workers. Test results should be made available to ordering physicians (EC and/or OHS) within 2 hours. This is to permit prompt dispensing of antiviral chemoprophylaxis to health care workers.
  • Hepatitis B Virus can be found in blood, saliva, semen and other body fluids of infected individuals. The virus is spread by direct contact with infectious body fluids.
  • Hepatitis C Virus can be found in blood and other body fluids of infected individuals. The virus is spread by direct contact with infectious body fluids.

CPT Codes

86703, 86803, 87340.


Last Updated


Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.