Lab Test

Donor ABO with A Subgroup

Donor ABO with A Subgrouping

Test Codes

EPIC: LAB5290, Beaker: DABAO


Blood Bank

Specimen Collection Criteria

Collect: One Pink-top EDTA tube, properly labeled. (Minimum Volume: 5.0 mL)

It is extremely important that blood samples are accurately labeled. Inpatient labels must include the following information:

  1. Patient's Name: Complete name, last name first. If the patient uses a first initial followed by a middle name, both must appear in proper order on the label.
  2. Wrist Band Number: Include the prefix letters, 4 numbers, and a suffix letter (example: BR1234T). Specimens lacking this complete information cannot be used for blood transfusion purposes.
  3. Hospital Identification Number: The seven-digit medical record number must be included on the label.
  4. Date and Time Collected
  5. Identification of Collector: Employee ID number must be on the sample tube or documented in the computer system.

Physician Office/Draw Specimen Preparation

Maintain specimen at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F) or refrigerated (2-8°C or 36-46°F).

Rejection Criteria

  • Improperly labeled specimens.
  • Specimens with inadequate volume.
  • Specimens collected in SST tubes.
  • Specimens contaminated with IV fluids.
  • Hemolyzed specimens will be tested only in emergency situations. 

In-Lab Processing

Centrifuge the specimen upon receipt in the Laboratory.


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 72 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): Specimens are held for a minimum of 7 days.

*Once a sample arrives in Blood Bank, it cannot be shared with other laboratories.


Royal Oak Transfusion Medicine Laboratory (Blood Bank)
Farmington Hills Transfusion Medicine Laboratory (Blood Bank)
Grosse Pointe Transfusion Medicine Laboratory (Blood Bank)
Troy Transfusion Medicine Laboratory (Blood Bank)
Dearborn Transfusion Medicine Laboratory (Blood Bank)
Taylor Transfusion Medicine Laboratory (Blood Bank)
Trenton Transfusion Medicine Laboratory (Blood Bank)
Wayne Transfusion Medicine Laboratory (Blood Bank)


Sunday – Saturday, 24 hours a day.
Results available within 8 hours of receipt in the Blood Bank for outpatient specimens.

Test Methodology



By report. The A Subgroup is performed only if the patient is Group A or AB.

Clinical Utility

ABO compatibility is the most important factor determining the immediate survival of kidney transplants. Because ABO antigens are expressed on all cells of the body, transplanted tissue is in continuous contact with the recipient's ABO antibodies. ABO compatibility is the same for kidney transplants as it is for blood; however, in some cases group A2 donor kidneys can be transplanted into group O recipients.


AABB Technical Manual, current edition.

CPT Codes

86900 (ABO), 86905 (Subgrouping).


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