Legionella Culture, Legionnaires Disease, Pontiac Fever, Pneumophila
Specimen Collection Criteria
Collect: One of the following specimen types, in a sterile, screw-capped collection container:
- Bronchial Brushings, Bronchial Washings, or Bronchoalveolar Lavage (BAL).
- Autopsy specimens from the chest cavity.
- Lung tissue.
- Thoracentesis fluid.
- Sputum (least preferred specimen type).
Physician Office/Draw Specimen Preparation
Deliver specimen to the Laboratory immediately after collection, refrigerated (2-8°C or 36-46°F), in a sterile, screw-capped collection container.
Preparation for Courier Transport
Transport: All specimen types, in a sterile, screw-capped collection container, refrigerated (2-8°C or 36-46°F).
- Specimens submitted in saline.
- Specimens submitted in containers with formalin or that ever contained formalin.
- Specimens in non-sterile or leaking containers.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 8 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days
Dearborn Microbiology Laboratory
Taylor, Trenton and Wayne sent to Dearborn Microbiology Laboratory for testing.
Royal Oak Microbiology Laboratory
Farmington Hills, Grosse Pointe, and Troy sent to Royal Oak Microbiology Laboratory for testing.
Sunday – Saturday, 24 hours a day.
Final negative results available within one week.
Identification of Legionella spp. from respiratory specimens provides a definitive diagnosis of Legionnaires disease.
This test is useful for the diagnosis of Legionnaires disease.
Pasculle, A.W., and McDevitt, D., 2007. Legionella Cultures. Clinical Microbiology Procedure Manual, 3rd Edition. ASM Press. Washington, D.C., Section 3.11.4.
Microbiology Laboratory – DBN
Microbiology Laboratory – RO
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