Cortisol, Free, 24 Hour Urine
Free Cortisol, Urine
WARDE: UFCO1, EPIC: LAB6979, SOFT: XCFUW
- Please refer to the Specimen Collection Manual for instructions on 24 Hour Urine Collection:
- If a random sample for Free Urine Cortisol is requested, please see Cortisol, Free, Urine, Random.
Specimen Collection Criteria
Collect: 24 hour urine sample with no preservative.
- Keep 24 hour urine specimen iced or refrigerated during collection.
- Include start and end dates and times for the collection period on the specimen container.
Urine Preservative Options
6N Hydrochloric Acid
Boric Acid (10g)
50% Acetic Acid
Physician Office/Draw Specimen Preparation
Maintain specimens refrigerated (2-8°C or 36-46°F) prior to transport.
Preparation for Courier Transport
Transport: Entire 24 hour urine collection, refrigerated (2-8°C or 36-46°F).
Specimens not collected and processed as indicated.
Measure total volume of 24 hour urine specimen. Record total volume and collection start and end dates and times in the LIS system. Aliquot 10.0 mL from the well-mixed 24 hour urine collection. (Minimum: 2.0 mL)
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 14 days
Specimen Storage in Department Prior to Disposal:
Specimen retention time is determined by the policy of the reference laboratory. Contact the Sendout Laboratory with any questions.
Sent to Warde Medical Laboratory, Ann Arbor, MI.
Results are available in 3-6 days.
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
Preferred screening test for Cushing Syndrome. Diagnosis of pseudo-hyperaldosteronism due to excessive licorice intake.
This test has limited usefulness in the evaluation of adrenal insufficiency.
Send Outs Laboratory – RO
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