Cell Counts (Cerebrospinal Fluid and Other Body Fluids)
CSF: Antrim #20735, EPIC: LAB5243 Body Fluids: Antrim #20744, EPIC: LAB5244
Specimen Collection Criteria
Collect: 1.0 mL Cerebrospinal Fluid (CSF) in a sterile collection container. (Minimum: 1.0 mL)
Also acceptable: 1.0 mL other body fluid (pleural, peritoneal, synovial, pericardial, etc.) in a sterile collection container. (Minimum: 1.0 mL)
- Cerebrospinal Fluid (CSF)
- In a sterile container collect 1 mL Cerebrospinal Fluid (CSF) (Minimum: 1.0 mL)
- No anticoagulant should be added to CSF.
- In a Lumbar Puncture Kit (includes tubes embossed 1-4) collect 1 mL in tubes in numeric order.
- The last tube collected should be submitted for the cell count (preferably tube 3 or 4).
- Other Body Fluids
- 1 mL other body fluid (pleural, peritoneal, synovial, pericardial, etc.) in a sterile collection container. (Minimum: 1.0 mL)
- Non-CSF body fluids that have a high probability of clotting should be collected in a tube containing anticoagulant. A Lavender-top EDTA tube is preferred.
Any request that is received with less than 0.5 mL of sample is not guaranteed, but a cell count will be attempted.
Physician Office/Draw Specimen Preparation
Arrange for transportation to the Laboratory prior to specimen collection. Do not centrifuge. Cell counts are most accurate if performed within 1 hour of collection. Maintain specimen refrigerated (2-8°C or 36-46°F) prior to transport.
Preparation for Courier Transport
Transport: 1.0 mL CSF or other body fluid, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)
- Clotted specimens.
- Specimens that have been centrifuged.
- Specimens received in bags, bottles, or syringes.
- Specimens which are not from a sterile body cavity (e.g. drainage, abscess, pus, etc.)
Inpatient Specimen Preparation
Transport specimen to the Laboratory immediately after collection.
Do not centrifuge. Cell counts are most accurate if performed within one hour after being drawn. Fluids greater than 24 hours old should not be analyzed for cell counts due to deterioration and/or lysis of cells.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 1 hour
Refrigerated (2-8°C or 36-46°F): 1 hour
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 30 days
Dearborn Hematology Laboratory
Farmington Hills Hematology/Coagulation Laboratory
Grosse Pointe Hematology/Urinalysis Laboratory
Royal Oak Hematology Laboratory
Taylor Hematology Laboratory
Trenton Hematology Laboratory
Troy Hematology/Coagulation Laboratory
Wayne Blood Bank
Sunday – Saturday, 24 hours a day.
STAT results available within 60 minutes if no further verification is necessary; 2 hours if verification is needed.
Routine results are available within 3 hours.
| Pleural Fluid:
|| WBC: Less than or equal to 1000 /mcL
|| RBC: Less than 10,000 /mcL*
| Spinal Fluid (CSF):
|| WBC: 0-5 /mcL
|| RBC: Absent
| Synovial Fluid:
|| WBC: 0-200 /mcL
|| RBC: Absent*
*RBC counts for non-CSF fluids will be reported as "less than 2000/mcL" when the RBC count is between 0-2000/mcL.
Manual microscopy, automated instrumentation as applicable.
- Cerebrospinal fluid may be examined as an aid in the diagnosis of meningitis, subarachnoid hemorrhage, encephalitis, CNS syphilis, CNS leukemia, CNS tumor and multiple sclerosis.
- In general, CSF changes due to a viral etiology will exhibit increased lymphocytes and those changes due to a bacterial etiology will show increased neutrophils. Other body fluids may be examined to determine the etiology of effusions and can sometimes be of extreme usefulness in distinguishing an inflammatory from malignant etiology.
Normal body fluids are clear, colorless and hypocellular. A change in physical appearance, an increase in cell count and differentiation of WBC may indicate a variety of diseases.
Hematology/Coagulation Laboratory – TRN
Hematology Laboratory – TYL
Blood Bank Laboratory – WYN
Hematology Laboratory – DBN
Hematology Laboratory – RO
Hematology/Coagulation Laboratory – FH
Hematology/Urinalysis Laboratory – GP
Hematology/Coagulation Laboratory – TR
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