Bullous Pemphigoid BP180 Antibody

BP 180, BP180 and IgG Antibodies

Test Codes

EPIC: LAB1232082, Beaker: BP180, Quest: 16034

Department

Send Outs

Specimen Collection Criteria

Collect: One plain Red-top tube. 

Also acceptable: One Gold-top SST tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to plastic transport tube and freeze (-20°C/-4°F or below) prior to transport.

Preparation for Courier Transport

Transport: 1.0 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.3 mL)

Rejection Criteria

• Gross hemolysis.
• Grossly lipemic.
• High levels of icterus.
• High levels of microbial growth.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to plastic transport tube and freeze (-20°C/-4°F or below) prior to transport.

Transport: 1.0 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.3 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 4 days
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Monday – Friday.
Results available within 2-6 days.

Reference Range

Negative	≤14 U/mL
Positive	≥15 U/mL

Test Methodology

Enzyme-Linked Immunosorbent Assay (ELISA).

CPT Codes

83520

Contacts

Last Updated

12/17/2025