Brucella Antibody Screen, IgM and IgG, EIA with reflex to Agglutination

Brucella Antibody (Total) by Agglutination

Test Codes

EPIC: LAB1231967, Beaker: Brucella AB, Quest: 91068

Department

Send Outs

Instructions

If Brucella IgM is ≥1.10, then Brucella Antibody, Agglutination will be performed at an additional charge (CPT code(s): 86622).

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to plastic transport tube and maintain at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: 1.0 mL serum, , room temperature (20-26°C or 68-78.8°F). (Minimum: 0.5 mL)

Rejection Criteria

• Hemolyzed specimens.
• Severely lipemic specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to plastic transport tube and maintain at room temperature (20-26°C or 68-78.8°F) prior to transport.

Transport: 1.0 mL serum, room temperature (20-26°C or 68-78.8°F). (Minimum: 0.5 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 7 days
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Sunday, Tuesday, Thursday.
Results available in 2-7 days.

Reference Range

Brucella IgG    <0.80 
Brucella IgM   <0.80

Interpretive Criteria
<0.80           Antibody Not Detected
0.80-1.09    Equivocal
≥1.10           Antibody Detected

Test Methodology

Immunoassay (IA)

Clinical Utility

Brucella Antibodies (IgG, IgM), EIA with Reflex to Agglutination – Detection of antibodies to Brucella sp. Usually indicates recent or past exposure to the organism.

CPT Codes

86622

Contacts

Last Updated

11/19/2025