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Bone Marrow Karyotype

Karyotyping, Chromosome Analysis, Karyogram, Bone Marrow, Bone Core Biopsy, genetic, GBMGT, cytogenetics

Test Codes

EPIC: LAB6460

Department

Cytogenetics

Specimen Collection Criteria

Collect: 2.0 mL bone marrow aspirate in a Dark Green-top Sodium Heparin tube. (Minimum: 1.0 mL)

• Bone marrow aspirates should be collected by a physician or trained technologist.
• Gently invert the tube to mix the specimen.
• A copy of the requisition must be sent with the specimen. The requisition form should contain the patient name, birth date, medical record number, requesting physician, specimen type and test request, date and time of collection, and tentative diagnosis.
• All specimens requiring FISH testing should identify the type of testing and tentative patient diagnosis on the requisition form. Both the FISH testing and conventional cytogenetics testing can be performed on the same specimen.

Physician Office/Draw Specimen Preparation

Do not freeze specimen. Maintain specimens at room temperature (20-25°C or 68-77°F) prior to courier pickup. For delays in transport (greater than 24 hours from the time of collection), refrigerate (2-8°C or 36-46°F) the specimen.

Preparation for Courier Transport

Transport: 2.0 mL bone marrow aspirate, at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL)

Rejection Criteria

• Specimens arriving in the Laboratory 4 days or more following the original collection date.
• Clotted and sub-optimal specimens (volume less than 0.5 mL) will be cultured but the Laboratory cannot guarantee a final result.

Inpatient Specimen Preparation

Hematology will notify the Cytogenetics Laboratory of specimen pickups at extension 89030.

In-Lab Processing

Specimens are processed immediately upon delivery to the Cytogenetics Laboratory. Pediatric and STAT specimens may be given a priority in processing and analysis to provide a rush result.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days. A backup cell pellet is maintained for three weeks past sign-out of the final report.

Laboratory

Royal Oak Cytogenetics Laboratory

Performed

Monday – Friday, 8:00 am – 5:00 pm.
Results available within 7-10 days of receipt in the Laboratory.
STAT analysis must be arranged in advance with the Laboratory.

Reference Range

Normal results would be 46,XX or 46,XY with no apparent abnormalities present.

Test Methodology

High resolution microscopic analysis of chromosomes to provide a digital image karyotype. Special stains or techniques such as C-Banding, NOR Banding and FISH may be required to further identify specific chromosomal abnormalities or variants. 

Interpretation

Based on completion of chromosomal analysis. Interpretation will be issued in the form of a final report.

Clinical Utility

Karyotyping (chromosome analysis) is performed on oncologic specimens to determine the presence of chromosomal abnormalities. Bone marrow aspirates are obtained to identify and characterize chromosomal involvement in the disease process, to stage or confirm a diagnosis, and to provide post-treatment follow up information to physicians.

Clinical Disease

To aid in a complete treatment plan for patients, abnormal karyotype results may need to be followed up at a later date to aid in the staging and progression of the disease process.

CPT Codes

88237, 88262, 88280.

Contacts

Last Updated

12/30/2022