Biotin (Vitamin B7)

Test Codes

EPIC: LAB12320762, Beaker: VIT B7, Quest: 391

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube. 

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30 minutes then centrifuge to separate serum from cells. Transfer serum to an amber plastic transport tube to protect from light and freeze (-20°C/-4°F or below) immediately.

Preparation for Courier Transport

Transport: 2.0 mL serum, protected from light, frozen (-20°C/-4°F or below). (Minimum: 1.0 mL)

Rejection Criteria

• Specimens not collected and processed as indicated.
• Specimens are grossly hemolyzed.
• Specimens are grossly lipemic.
• Lipemic specimens.
• Not protected from light.
• Samples other than serum.

In-Lab Processing

Let specimen clot 30 minutes then centrifuge to separate serum from cells. Transfer serum to an amber plastic transport tube to protect from light and freeze (-20°C/-4°F or below

Transport: 2.0 mL serum, frozen (-20°C/-4°F or below) immediately.

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 14 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Varies.
Results available in 4-6 days.

Reference Range

<12 years	100.0-2460.2 pg/mL
≥12 years	221.0-3004.0 pg/mL

Test Methodology

Bioassay (BA)

CPT Codes

84591

Contacts

Last Updated

12/17/2025