Amyloidosis/Neuropathy Monoclonal Gammopathy Evaluation
Test Codes
EPIC: LAB8573, Beaker: NMGE
Department
Special Chemistry
Specimen Collection Criteria
Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)
Physician Office/Draw Specimen Preparation
Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transfer serum to a plastic aliquot tube and refrigerate (2-8°C or 36-46°F) within 8 hours of collection. (Minimum Serum: 0.5 mL)
Preparation for Courier Transport
Transport: Serum aliquot tube, refrigerated (2-8°C or 36-46°F). (Minimum Serum: 0.5 mL)
Rejection Criteria
Plasma specimens.
Severely hemolyzed, lipemic or icteric specimens.
In-Lab Processing
Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transfer serum to a plastic aliquot tube. Room temperature is acceptable for a maximum of 8 hours. (Minimum Serum: 0.5 mL)
Storage
Specimen Stability for Testing:
Centrifuged SST Tubes
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable
Red-top Tubes, and Microtainers®
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable
Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 8 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 1 month
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days
Laboratory
Royal Oak Special Chemistry Laboratory
Performed
Monday – Friday.
Results available within 3 business days.
Reference Range
See the Chemistry Department’s reference ranges for Total Protein.
See Free Light Chains’ reference ranges for Free Kappa and Free Lambda.
Age | Albumin | Alpha-1 Globulins | Alpha-2 Globulins | Beta Globulins | Gamma Globulins |
0-6 Days | 2.19-3.26 | 0.18-0.51 | 0.26-0.62 | 0.35-0.56 | 0.81-1.67 |
7-15 Days | 3.41-4.80 | 0.18-0.51 | 0.26-0.62 | 0.35-0.56 | 0.81-1.67 |
16 Days-12 Months | 3.41-4.80 | 0.18-0.51 | 0.43-0.94 | 0.43-0.84 | 0.58-1.55 |
13 Months-5 Years | 3.41-4.80
| 0.18-0.68 | 0.43-1.25 | 0.43-1.03 | 0.58-2.03 |
6-16 Years | 3.50-5.09 | 0.18-0.68 | 0.43-1.25 | 0.43-1.03 | 0.58-2.03 |
17 Years and older | 3.42-4.86 | 0.22-041 | 0.55-1.09 | 0.53-1.01 | 0.60-1.45 |
Monoclonal protein: Negative
Test Methodology
Capillary Protein Electrophoresis and Immunofixation Electrophoresis.
Interpretation
Capillary Electrophoresis is performed with quantitation of any monoclonal protein detected. A complete immunofixation is also performed. Immunoglobulin quantitations are performed on initial diagnosis of a monoclonal protein, and on follow-up when quantitation by scan is not possible. Immunoglobulin quantitation is also performed with initial diagnosis of hypogammaglobulinemia. If only a small amount of monoclonal protein is present in the beta globulin region, quantitation may not be possible and following the affected immunoglobulin level is the best way to monitor a patient. If the patient has a free kappa or lambda monoclonal protein, use of the free light chain assay to monitor the monoclonal protein quantitation, is recommended.
Clinical Utility
Screening/monitoring of patients with monoclonal proteins, which can be associated with various plasma cell (MGUS, myeloma, light chain amyloidosis) and other hematolymphoid neoplasms.
CPT Codes
83520 x 2, 84165 and 86334.
Contacts
Special Chemistry Laboratory – RO
248-551-8044
Name: Special Chemistry Laboratory – RO
Location:
Phone: 248-551-8044
Last Updated
3/9/2023
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