Amylase Isoenzymes

Salivary Amylase, Amylase Iso Enzymes, Amylase Pancreatic, Fractionated Amylase

Test Codes

EPIC: LAB1232139, Beaker: Amylase Iso, Quest: 845

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: 1.0 mL serum, at room temperature (20-26°C or 68-78.8°F). (Minimum: 0.5 mL)

Rejection Criteria

• Hemolyzed specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport at room temperature (20-26°C or 68-78.8°F).

Transport: 1.0 mL serum, at room temperature (20-26°C or 68-78.8°F). (Minimum: 0.5 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 7 days
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 21 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Sunday, Tuesday, Thursday.
Results available in 3-6 days.

Reference Range

Amylase	21-101 U/L
Pancreatic Isoenzyme	16-46 U/L
Salivary Isoenzymes	4-61 U/L

Test Methodology

Enzymatic

Clinical Utility

Amylase measurements are used primarily for the diagnosis and treatment of pancreatitis. The elevation of a-amylase activity in serum, however, is not specifically indicative of a pancreatic disorder since the enzyme is also produced by the salivary glands and other organs. Clinical evaluations of patients with acute pancreatitis have shown that pancreatic isoamylase has a greater sensitivity than total amylase.

CPT Codes

82150x2.

Contacts

Last Updated

12/17/2025