Amitriptyline Level (including Nortriptyline)
Elavil, Pamelor, Aventyl, Amitriptyline (including Nortriptyline) Level
Antrim #17253, EPIC: LAB5373, SOFT: AMITR
Specimen Collection Criteria
Collect: One plain Red-top tube. (Minimum Whole Blood: 2.0 mL)
Do not use Serum Separator Tubes.
Time of Collection: Trough, just prior to the next dose.
Record the exact time of specimen collection on the tube or in the computer system.
Physician Office/Draw Specimen Preparation
Let specimens clot 30-60 minutes. Centrifuge specimen to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum: 0.5 mL serum)
Preparation for Courier Transport
Transport: Centrifuged collection tube, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL serum)
- Serum Separator (SST) tubes.
- Severely lipemic or hemolyzed specimens.
Let specimens clot 30-60 minutes. Centrifuge specimen to separate serum from cells. Deliver immediately to the appropriate testing station.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 3 months
Specimen Storage in Department Prior to Disposal:
Frozen (-20°C/-4°F or below): 1 month
Royal Oak Toxicology Laboratory
Results available the same day as testing performed (1-4 days).
Amitriptyline + Metabolite: 125-250 ng/mL.
Critical: Greater than 500 ng/mL total.
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS).
This test was developed and its performance characteristics determined by Beaumont Health. It has not been cleared or approved by the FDA. The Laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes and should not be regarded as investigational or for research.
Tricyclic antidepressants, when present in high concentrations, or when administered to elderly or susceptible populations, may depress cardiac function.
Amitriptyline is a tricyclic antidepressant. It is administered for the relief of symptoms of endogenous depression. Amitriptyline is metabolized to nortriptyline. This assay is used to monitor the therapeutic drug level and evaluate the toxicity of amitriptyline and nortriptyline.
80355 (alternate: G0480).
LOINC: 3335-7, Amitriptyline-16225-5, Nortriptyline-61427-1
Toxicology Laboratory – RO
Microtainer® and Vacutainer® are registered trademarks of Becton, Dickinson and Company.
UroVysion® is a registered trademark of Abbott Laboratories. ThinPrep® is a registered trademark of Hologic, Incorporated.