Aminolevulinic Acid, 24 Hour Urine
ALA (Aminolevulinic Acid), DALA (Delta-Aminolevulinic Acid), AIP (Acute Intermittent Porphyria), ALA Dehydratase Deficiency Porphyria (ADP), HCP (Hereditary Coproporphyria), VP (Variegate Porphyria)
EPIC: LAB6747, Beaker: XALUA, Mayo: FALAU
- A Urine Creatinine test is performed on each sample.
- Please refer to the Specimen Collection Manual for instructions on 24-Hour Urine Collection:
- The patient should refrain from alcohol consumption at least 24-hours prior to start of collection.
Specimen Collection Criteria
Collect (preferred specimen): 24-hour urine sample with no preservative. Keep 24-hour urine specimen iced or refrigerated during collection.
- Include start and end dates and times for the collection period on the specimen container.
Urine Preservative Options
6N Hydrochloric Acid
Boric Acid (10g)
50% Acetic Acid
Physician Office/Draw Specimen Preparation
Maintain specimens refrigerated (2-8°C or 36-46°F) prior to transport
Preparation for Courier Transport
Transport: Entire 24-hour urine collection, refrigerated (2-8°C or 36-46°F)
Specimens not collected and processed as indicated.
Measure total volume of 24-hour urine specimen. Record total volume and collection start and end dates and times in the LIS system. Aliquot 4.0 mL from the well-mixed 24-hour urine collection and transfer to a transport tube. (Minimum: 1.2 mL)
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 4 days
Frozen (-20°C/-4°F or below): 30 days
Specimen Storage in Department Prior to Disposal:
Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.
Sent to Mayo Clinic Laboratories in Rochester, MN. Forwarded to ARUP Laboratories, Salt Lake City, UT.
Monday, Wednesday, Friday.
Results available within 2-9 days
Quantitative Ion Exchange Chromatography / Spectrophotometry.
- Slight elevations of ALA may be seen in association with exposure to alcohol, lead, and a variety of other agents. Significant elevations are often associated with the acute porphyrias (massive elevations of porphobilinogen, PBG). Significant elevations are also seen in hereditary tyrosinemia.
- Specimen preservation with acid or base is discouraged and may cause assay interference. When collecting the urine for additional tests that require acid or base preservation, the ALA aliquot should be taken prior to the addition of the acid or base.
- Aminolevulinic acid (ALA) dehydratase is poisoned by heavy metals, such as lead and mercury, so that ALA accumulates, and excess amounts appear in the urine.
- In children, measurement of urinary ALA is more sensitive than measurement of blood lead for evaluation of lead poisoning.
- ALA excretion may also be increased in congenital hepatic porphyria.
- ALA may be elevated in hereditary tyrosinemia and late pregnancy.
The ALA assay aids in the diagnosis of a form of acute intermittent porphyria or an induction/toxic porphyria. This assay indicates lead intoxication in children.
LOINC: 34284-0, 14689-4
Send Outs Laboratory – RO
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