Alpha Fetoprotein, Amniotic Fluid
Amniotic Fluid-AFP, Alphafetoprotein- Amniotic Fluid, Alphafetoprotein, Amniotic Fluid
EPIC: LAB5798, SOFT: AFPAF
An "Amniotic Fluid AFP" requisition form should be sent with each sample. This must include the following information:
- Reason for amniocentesis.
- Ultrasound information on gestational age.
Specimen Collection Criteria
Collect: 2.0 mL amniotic fluid in a sterile collection container. (Minimum: 1.5 mL)
Physician Office/Draw Specimen Preparation
Refrigerate (2-8°C or 36-46°F) specimen after collection and arrange for transportation to the Laboratory immediately. Room temperature is acceptable for a maximum of 4 hours. (Minimum: 1.5 mL)
Preparation for Courier Transport
Transport: 2.0 mL amniotic fluid, refrigerated (2-8°C or 36-46°F). (Minimum: 1.5 mL)
Specimens not collected and processed as indicated.
Inpatient Specimen Preparation
Send specimen to the Laboratory for processing immediately after collection. (Minimum: 1.5 mL)
Refrigerate specimen prior to testing. Room temperature is acceptable for a maximum of four hours. (Minimum: 1.5 mL)
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 4 hours
Refrigerated (2-8°C or 36-46°F): 4 months
Frozen (-20°C/-4°F or below): 3 years
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days
Royal Oak Special Testing Laboratory
Samples with an abnormal result or abnormal history will be automatically sent to Mayo in Minnesota.
Monday – Friday.
Results available within 2 business days.
The AFP result is reported as a MoM (multiple of the median) - this is calculated for the appropriate gestational age. When the MoM is greater than 1.5 an abnormality may be present. Such a sample needs additional testing (repeat AFP and acetylcholinesterase). Repeat testing is sent to Mayo (test code:ACHE).
ARUP uses Qualitative Gel Electrophoresis/Radial Immunodiffusion.
This assay aids in evaluating a patient's pregnancy for neural tube defects. The assay may be ordered if a patient has an elevated serum AFP or an abnormal ultrasound. It may be appropriate in patients with insulin-dependent diabetes mellitus or those taking valproic acid.
Special Chemistry Laboratory – RO
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