Aggressive B-cell Lymphoma Panel by Fluorescence In Situ Hybridization (FISH) Analysis
FISH, genetic, double hit lymphoma, triple hit lymphoma, B-cell lymphoma, High-Grade B-Cell Lymphoma, with MYC and BCL2, and/or BCL6 Rearrangements (Aggressive B-cell Lymphoma, IgK (lg Kappa chain) gene rearrangement for Burkitt’s lymphoma, IgL (lg Lambda chain) gene rearrangement for Burkitt’s lymphoma, MYC gene rearrangement for Burkitt’s lymphoma-associated translocations, IgH/MYC fusion for t(8;14) detection
Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC is needed. EPIC: LAB7113
Specimen Collection Criteria
Collect: Bone marrow aspirate in a Dark Green-top Sodium Heparin tube, lymph node touch preparation, or paraffin-embedded tissue section.
A copy of the requisition must be sent with the specimen.
Physician Office/Draw Specimen Preparation
Do not freeze specimen. Maintain specimens at room temperature (20-25°C or 68-77°F) prior to courier pickup. For delays in transport (greater than 24 from the time of collection), refrigerate (2-8°C or 36-46°F) the specimen.
Preparation for Courier Transport
Transport: Bone marrow, lymph node touch preparation, or paraffin-embedded tissue, at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F).
Specimens arriving in the Laboratory 4 days or more following the original collection date.
Frozen or fixed specimens.
Specimens are processed immediately upon receipt in the Laboratory.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days. A backup cell pellet is maintained for three weeks after the case has been signed out.
Royal Oak Cytogenetics Laboratory
Monday – Friday, 8:00 am – 5:00 pm.
Results available within 7 days of receipt in the Laboratory.
Positive or negative for a neoplastic clone. An interpretative report will be provided.
Fluorescence In Situ Hybridization (FISH) Analysis.
A positive FISH result indicates the presence of MYC, IGH/BCL2, and/or BCL6 gene rearrangement.
The presence in a B-cell lymphoma of MYC gene rearrangement plus either BCL2 or BCL6 rearrangement defines a double-hit lymphoma and predicts a poor prognosis that requires aggressive treatment. This assay can be performed on a bone marrow biopsy, lymph
node touch preparation, or a paraffin-embedded tissue section.
For MYC Gene Rearrangement: 88271x1, DNA probe, each.
For IGH/MYC: 88271x3.
For Ig Kappa or Ig Lambda: 88271x1, DNA probe, each.
For All Assays: 88275x1, Interphase analysis, 100-300 cells.
Cytogenetics Laboratory – RO
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