21-Hydroxylase Antibody

Test Codes

EPIC: LAB12320756, Beaker: 21-Hydroxyl, Quest: 39226

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transport serum to a plastic transport tube, maintain at room temperature (20-26°C or 68-78.8°F) prior to transport.

Preparation for Courier Transport

Transport: 0.5 mL serum, room temperature (20-26°C or 68-78.8°F). (Minimum: 0.3 mL)

Rejection Criteria

• Grossly Hemolysis.
• Grossly Icteric specimens.
• Specimens not collected and processed as indicated.

Inpatient Specimen Preparation

Deliver specimen to Laboratory immediately after collection for processing.

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Transport serum to a plastic transport tube, refrigerated at (2-8°C or 36-46).

Transport: 0.5 mL serum, at room temperature (20-26°C or 68-78.8°F). (Minimum: 0.3 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 5 days
Refrigerated (2-7°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 60 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Wednesday.
Results available in 4-11 days.

Reference Range

Negative.

Test Methodology

Enzyme-linked Immunosorbent Assay (ELISA).

Clinical Utility

21-Hydroxylase Antibody is present in patients with autoimmune destruction of the adrenal glands, leading to Addison's disease. Antibody is also seen in autoimmune polyglandular syndrome.

CPT Codes

83516

Contacts

Last Updated

12/17/2025