17-Hydroxypregnenolone

Hydroxypregnenolone, 17-, Pregnenolone, 17-OH-

Test Codes

EPIC: LAB1232056, Beaker: 17-Hydrox S, Quest: 8352

Department

Send Outs

Specimen Collection Criteria

Collect: One plain Red-top tube.

Unacceptable: One Gold-top Serum Separator Tube (SST).

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and refrigerate at (2-8°C or 36-46) prior to transport.

Preparation for Courier Transport

Transport: 2.0 mL serum, refrigerated at (2-8°C or 36-46). (Minimum: 0.4 mL)

Rejection Criteria

• Gross hemolysis.
• Grossly lipemic.
• Grossly icteric.
• Serum separator tube (SST).
• Received at room temperature.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to a plastic transport tube and maintain refrigerated at (2-8°C or 36-46) prior to transport..

Transport: 2.0 mL serum, refrigerated at (2-8°C or 36-46). (Minimum: 0.4 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 5 days
Frozen (-20°C/-4°F or below): 28 days

Specimen Storage in Department Prior to Disposal: 14 days

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Sunday – Friday.
Results available in 2-5 days.

Reference Range

By report.

Test Methodology

Chromatography / Mass Spectrometry

Interpretation

Reference Range(s):

Adult Males

≤700 ng/dL

Adult Females, Pre-Menopausal:

Mid Follicular	38-378 ng/dL
Surge	33-583 ng/dL
Mid Luteal	70-470 ng/dL

Pediatric Male and Female:

<30 days	≤3013 ng/dL
1-11 months	≤869 ng/dL
1 year	≤557 ng/dL
2 years	≤487 ng/dL
3 years	≤541 ng/dL
4 years	≤561 ng/dL
5 years	≤564 ng/dL
6 years	≤561 ng/dL
7 years	≤549 ng/dL
8 years	≤569 ng/dL
9 years	≤590 ng/dL
10 years	≤594 ng/dL
11 years	≤629 ng/dL
12 years	≤666 ng/dL
13 years	≤678 ng/dL
14 years	≤706 ng/dL
15 years	≤735 ng/dL
16 years	≤739 ng/dL
17 years	≤756 ng/dL

Clinical Utility

17-Hydroxypregnenolone is useful in the diagnosis of 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.

CPT Codes

84143

Contacts

Last Updated

3/9/2026