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Myositis Autoantibodies

PL-7, PL-12, Mi-2, Ku, EJ, OJ, SRP, U3-RNP, U2-RNP, Jo-1, TIF-1gamma, MDA-5 (CADM-140), NXP-2 (P140), PM/Scl-100, SS-A 52kD, U1-RNP, Myositis Panel 3, Myomarker 3, Myositis Assessor 

Test Codes

EPIC: LAB6501, Beaker: XMA, Mayo: FMYO3

Department

Send Outs

Specimen Collection Criteria

Collect: Two plain Red-top tubes.

Also acceptable: Two Gold-top SST tubes.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to two plastic transport tubes and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport:  5.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 4.0 mL – volume does not allow repeat testing) 

Rejection Criteria

• Gross hemolysis and lipemia.
• Gross Icteric specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Transfer serum to two plastic transport tubes and refrigerate (2-8°C or 36-46°F). 
 
Transport: 5.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 4.0 mL- volume does not allow repeat testing) 

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 7 days
Refrigerated (2-8°C or 36-46°F): 14 days
Frozen (-20°C/-4°F or below): 60 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Mayo Clinic Laboratories in Rochester, MN. Forwarded to Esoterix Endocrinology.

Performed

Varies.
Results available in 2-3 weeks.

Reference Range

By report.

Test Methodology

Enzyme-linked immunosorbent assay (ELISA); RIPA Gel Radiography.

Clinical Utility

A high proportion of patients with idiopathic inflammatory myopathies (IIMs) which include polymyositis, dermatomyositis and myositis associated with other rheumatic diseases, have autoantibodies directed against aminoacyl-transfer RNA (tRNA) synthetases (myositis-specific antibodies), which are a group of cellular enzymes that catalyze the binding of one amino acid to its tRNA. Autoantibodies to eight of these synthetases, histidyl (Jo-1), threonyl RS (PL-7), alanyl RS (PL-12), isoleucyl RS (OJ), glycyl RS (EJ), leucyl RS, lysyl RS and GluPro RS are reported in IIMs. Histidyl-tRNA synthetase (Jo-1) antibodies are the most common (20-30% of IIMs). Anti-PL-7 and PL-12 are found in 3%, anti-EJ occur in 3%, OJ occur in 2%, and anti-GluPro RS, leucyl RS and lysyl RS occur in less than 1%. Aminoacyl-tRNA synthetase autoantibodies are strongly also associated with interstitial lung disease (ILD), arthritis and Raynaud phenomenon.

CPT Codes

83516 x9, 86235 x7.
LOINC:  PL-7  33772-5, PL-12  33771-7, EJ 45149-2, OJ 45152-6, SRP 33921-8, MI-2  18485-3, U3 RNP 49963-2

Contacts

Last Updated

5/8/2023