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Phospholipase A2 Receptor Antibody (PLA2R)

Test Codes

EPIC: LAB7163, Beaker: XPLA2, ARUP: 2011828

Department

Send Outs

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.

Also acceptable: One plain Red-top tube.

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Preparation for Courier Transport

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.2 mL)

Rejection Criteria

• Hemolyzed specimens.
• Hyperlipemic specimens.
• Icteric specimens.
• Specimens not collected and processed as indicated.

In-Lab Processing

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and refrigerate (2-8°C or 36-46°F).

Transport: 1.0 mL serum, refrigerated (2-8°C or 36-46°F). (Minimum: 0.2 mL)

Storage

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 2 weeks
Frozen (-20°C/-4°F or below): 1 year

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to ARUP Laboratories, Salt Lake City, UT.

Performed

Monday, Wednesday, Friday.
Results available in 2-7 days.

Reference Range

Less than 1:10.

Test Methodology

Semi-Quantitative Indirect Fluorescent Antibody.

Interpretation

A positive result (1:10 or greater) for Phospholipase A2 Receptor Antibody, IgG, in conjunction with other laboratory and clinical findings, supports a diagnosis of primary membranous glomerulonephropathy (pMGN).

If Phospholipase A2 Receptor Antibody, IgG is positive, then a Phospholipase Receptor A2 Antibody, IgG titer is reported. Additional charges apply.

Clinical Utility

Distinguishing primary from secondary membranous nephropathy.

Primary membranous glomerular nephropathy has been shown to be associated with immune complexes containing auto-antibodies to the podocyte protein M-type phospholipase A2 receptor on the glomerular basement membrane. There is evidence that levels of these antibodies correlate with disease activity and may be useful in predicting disease recurrence after transplantation.

Reference: Clin J Am Soc Nephrol 13:784-786, 2018.

CPT Codes

80299, 86256 if indicated.

Contacts

Last Updated

11/3/2023