C. cayetanensis, Cyclospora Stain
At least 2 weeks should elapse following barium administration before collection of the specimen.
Specimen Collection Criteria
Collect: Random stool specimen placed in a transport vial with SAF preservative.
- Fill the vial until the fluid level reaches the red fill line.
- Unpreserved stool must be placed in SAF within 1 hour of collection.
Physician Office/Draw Specimen Preparation
Maintain specimens in SAF preservative at room temperature (20-26°C or 68-78.8°F) prior to transport.
Preparation for Courier Transport
Transport: Stool in SAF preservative, at room temperature (20-26°C or 68-78.8°F).
- Solid or formed specimens.
- Rectal swabs.
- Specimens in leaking or soiled containers.
- Specimens containing oil or urine.
- Specimens that contain toilet water.
- Specimens received in diapers.
- Specimens collected within 7 days of the patient taking a laxative.
- Specimens collected following a soap suds enema.
- Stools containing barium from a previous radiological procedure.
- Specimens collected within 14 days following antibiotics.
- Unpreserved stool greater than 1 hour past collection.
- Specimens in PVA.
- Frozen specimens.
- Testing will not be performed on more than one specimen collected per patient within a 24-hour period.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 1 hour (Unpreserved)
Room Temperature (20-25°C or 68-77°F): 7 days (Preserved)
Refrigerated (2-8°C or 36-46°F): 7 days (Preserved)
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Room Temperature (20-26°C or 68-78.8°F): 7 days
Dearborn Microbiology Laboratory
Taylor, Trenton and Wayne sent to Dearborn Microbiology Laboratory for testing.
Royal Oak Microbiology Laboratory
Farmington Hills, Grosse Pointe, and Troy sent to Royal Oak Microbiology Laboratory for testing.
Monday – Friday, 7:00 am – 3:30 pm.
Results available within 24 hours.
No Cyclospora seen.
Concentration, stain, microscopic examination.
Cyclospora spp. are abundant in the stool of infected individuals.
This assay aids in the diagnosis of cyclosporiasis.
Organisms in stool previously named blue-green algae (cyanobacteriumlike bodies) or "large Cryptosporidium" have recently been identified as belonging to the coccidian genus Cyclospora. Infection with Cyclospora spp. is primarily seen in HIV-infected individuals and in immunocompetent adults and children. The most common characteristic of infection with this organism is prolonged, often relapsing, watery, non-bloody diarrhea that is frequently associated with weight loss. In some cases, bloating, fatigue, nausea, vomiting, crampy abdominal pain, and fever also occur. The mean duration of diarrhea has ranged from 19 to 43 days (1). Symptoms are more severe in AIDS patients, lasting up to 70 days (2).
Infection is frequently linked to contaminated water and occurs worldwide (1). Outbreaks in 1996 and 1997 were associated with imported berries, basil, and baby lettuce. Most cases were reported in April – August (2).
- Novak-Weekley, S., and A.L. Leber,. 2015. Cystoisospora, Cyclospora, and Sarcocystis, Jorgensen, J.H., et. al. (eds.), Manual of Clinical Microbiology, 11th ed. ASM Press, Washington, D.C.
Microbiology Laboratory – DBN
Microbiology Laboratory – RO
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