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RBC Antigen Typing/Phenotype

Rh (Ee, Cc, D), Red Blood Cell Phenotype, RBC Phenotype, RBC Antigen Typing

Test Codes

Fy (Duffy), EPIC: LAB5330; Kk (Kell), EPIC: LAB5292; Jk (Kidd), EPIC: LAB5293; MN, EPIC: LAB5294; Ss, EPIC: LAB5295; Rh (Ee, Cc, D), EPIC: LAB5296

Department

Blood Bank

Instructions

In order to run RBC Antigen Typing/Phenotyping on a father, the Blood Bank department must be supplied the mother's name, date of birth, and medical record number. Please enter as an order comment or call the Blood Bank department. 

Specimen Collection Criteria

Collect: One Pink-top EDTA tube, properly labeled. (Minimum Volume: 5.0 mL)
Also acceptable: (For Nursery Patients) One Pink-top EDTA Microtainer®, properly labeled. (Minimum Volume: 0.5 mL) 

It is extremely important that blood samples are accurately labeled. Inpatient labels must include the following information:

1. Patient's Name: Complete name. If the patient uses a first initial followed by a middle name, both must appear in proper order on the label.
2. Wrist Band Number:  Include the prefix letters, 4 numbers, and a suffix letter (example: BR1234T). Specimens lacking this complete information cannot be used for blood transfusion purposes and require recollection.
3. Hospital Identification Number: The seven digit medical record number must be included on the label.
4. Date and Time Collected
5. Identification of Collector: Employee ID number or first initial and complete last name must be on the sample tube or documented in the computer system.

For Beaumont Reference Laboratory (Outreach) patients, please refer to the Laboratory Bulletin on Laboratory Specimens: How to Label Correctly. 

Physician Office/Draw Specimen Preparation

Maintain specimen at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F) prior to transport.

Preparation for Courier Transport

Transport: Whole blood at room temperature (20-25°C or 68-77°F) or refrigerated (2-8°C or 36-46°F).

Rejection Criteria

• Improperly labeled specimens.
• Specimens with inadequate volume.
• Specimens collected in inappropriate collection tubes.
• Specimens contaminated with IV fluids.
• Grossly hemolyzed samples will be tested only in emergency situations. 

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 24 hours
Refrigerated (2-8°C or 36-46°F): 72 hours
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): Specimens are held for a minimum of 7 days.

*Once a sample arrives in Blood Bank, it cannot be shared with other laboratories.

Laboratory

Dearborn Transfusion Medicine Laboratory (Blood Bank)
Farmington Hills Transfusion Medicine Laboratory (Blood Bank)
Grosse Pointe Transfusion Medicine Laboratory (Blood Bank)
Royal Oak Transfusion Medicine Laboratory (Blood Bank)
Taylor Transfusion Medicine Laboratory (Blood Bank)
Trenton Transfusion Medicine Laboratory (Blood Bank)
Troy Transfusion Medicine Laboratory (Blood Bank)
Wayne Transfusion Medicine Laboratory (Blood Bank)

Performed

Monday – Friday, 6:30 am-3:00 pm. 
Results available within 24 hours.

Test Methodology

Hemagglutination.

Interpretation

By report. Invalid results may occur if the patient's red blood cells have a positive DAT (Direct Coombs) or recent transfusion history.

Clinical Utility

Blood group antigen typing is used in cases where a maternal clinically significant antibody exists to evaluate the potential for the father of the unborn child to pass on the antigen corresponding to the maternal antibody.

CPT Codes

86905

Contacts

Last Updated

10/29/2023