JC Polyoma Virus DNA, Quantitative, Real-Time PCR, CSF

Polyomavirus, JCV Polyoma, JCV Viral Load, JC Virus DNA,  Quant PCR,  CSF, JCV Virus DNA, QT Real-Time PCR

Test Codes

EPIC: LAB1232032, Beaker: JCPolyomaCSF, Quest: 16442

Department

Send Outs

Specimen Collection Criteria

Collect: 0.7 mL CSF in a leak-sterile container. (Minimum: 0.3 mL)

Physician Office/Draw Specimen Preparation

Transfer CSF into plastic transport tube and freeze (-20°C/-4°F or below) prior to transport.

Preparation for Courier Transport

Transport: 0.7 mL CSF, frozen (-20°C/-4°F or below). (Minimum: 0.3 mL)

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Transfer CSF into plastic transport tube and freeze (-20°C/-4°F or below) prior to transport.

Transport: 0.7 mL CSF in a leak-sterile container. (Minimum: 0.3 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 30 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to Quest Diagnostics, Wood Dale, IL.

Performed

Daily.
Results available in 2-4 days. 

Reference Range

JC Virus DNA, QN PCR, CSF	Not Detected (IU/mL)
JC Virus DNA, QN PCR, CSF	Not Detected (Log IU/mL)

Test Methodology

Real-Time Polymerase Chain Reaction

Clinical Utility

JC Polyoma Virus DNA, Quantitative, Real-Time PCR, CSF – JC Polyoma Virus is the cause of Progressive Multifocal Leukoencephalopathy (PML), a severe demyelinating disease of the central nervous system. PML is a particular concern for individuals infected with the human immunodeficiency virus. Quantification of JC virus DNA is based upon the real-time PCR amplification and detection of JCV genomic DNA. Reportable range is 50 to 50,000,000 IU/mL (1.70 to 7.70 Log IU/mL).

CPT Codes

87799

Contacts

Last Updated

11/24/2025