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Gliadin (Deamidated) Antibodies

Celiac disease, Gliadin Antibody Panel, tropical sprue, Gliadin Antibody, IgG, Gliadin Antibody, IgA, Gliadin Antibodies IgA, IgG

Test Codes

EPIC: LAB5760, SOFT: GLI2

Department

Special Chemistry

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)

Physician Office/Draw Specimen Preparation

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 0.5 mL)

Rejection Criteria

Plasma specimens.

Severely hemolyzed, lipemic or icteric specimens.   

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers^® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 2 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers^® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 2 days
Frozen (-20°C/-4°F or below): 3 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Wednesday.
Results available in 3 business days.

Reference Range

Gliadin IgA Ab & Gliadin IgG Ab

Negative: Less than 20 Units.
Weak Positive: 20-30 Units.
Moderate-Strong Positive: Greater than 30 Units.

Test Methodology

EIA.

Interpretation

IgA and IgG gliadin antibodies have moderate sensitivity for detection of celiac disease; however, their specificity is much less than tissue transglutaminase or endomysial antibodies and their positive predictive value in the general population is poor. It is not uncommon for normal persons and patients with GI inflammation to have positive results.

IgA gliadin antibody testing is useful for diagnosis in symptomatic children less than 2 years and may be useful in all ages if it is necessary to monitor dietary compliance. IgA gliadin antibody levels tend to normalize within 3-6 months of gluten withdrawal. IgG gliadin antibody testing is most useful in patients with selective IgA deficiency.

Clinical Utility

Gliadin antibody testing can be useful to monitor dietary compliance in celiac disease and may be useful for diagnosis of the disease. Note that histologic confirmation is required for a diagnosis of celiac disease. Patients should not begin a gluten-free diet until after intestinal biopsy has been performed and a diagnosis established.

CPT Codes

83520
LOINC: 20495-8, 20496-6

Contacts

Last Updated

1/11/2023