Acute Myeloid Leukemia Fluorescence In Situ Hybridization (FISH) Analysis
RUNX1T1/RUNX1 [ETO/AML1] fusion for t(8;21), CBFß/MYH11 gene for inv(16)/t(16;16), MLL gene rearrangement for t(11q23;var), PML/RARA for t(15;17), RPN1/MECOM, for inv(3)/t(3;3), Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC or SOFT is needed.
Specimen Collection Criteria
Collect: Peripheral blood (Min: 3.0 mL) or bone marrow aspirate (Min: 1.0 mL) in Dark Green-top Sodium Heparin tubes.
A copy of the requisition must be sent with the specimen.
FedEx Shipping Instructions
Transport 1-2 mL bone marrow or 5-7 mL whole blood (minimum: 3 mL) at room temperature. If the specimen will not be received at the testing laboratory within 48 hours of collection, transport refrigerated. Do not fix or freeze the specimen. A pathology report for the patient must be provided.
Read our complete shipping instructions.
Physician Office/Drawsite Specimen Preparation
Do not freeze specimen. Maintain specimens at room temperature (20-26°C or 68-78.8°F) prior to courier pickup. For delays in transport (greater than 48 from the time of collection), refrigerate (2-8°C or 36-46°F) the specimen.
Preparation for Courier Transport
Transport: 5-7 mL peripheral blood or 1-2 mL bone marrow, at room temperature (20-26°C or 68-78.8°F) or refrigerated (2-8°C or 36-46°F). (Min: 3.0 mL blood or 1.0 mL bone marrow)
- Specimens arriving in the Laboratory 3 days or more following the original collection date.
- Frozen or fixed specimens.
Specimen Stability for Testing:
Room Temperature (20-26°C or 68-78.8°F): 48 hours
Refrigerated (2-8°C or 36-46°F): 96 hours
Frozen (-20°C/-4°F or below): Unacceptable
Specimen Storage in Department Prior to Disposal:
Refrigerated (2-8°C or 36-46°F): 7 days. A backup cell pellet is maintained for three weeks after the case has been signed out.
Royal Oak Clinical Cytogenomics Laboratory.
Monday - Friday, 8:00 a.m. - 5:00 p.m.
Results available within one week of receipt in the Laboratory.
Positive or negative for a neoplastic clone. An interpretative report will be provided.
Fluorescence In Situ Hybridization (FISH) Analysis.
A positive FISH result indicates the presence of the chromosome abnormality. The presence of RUNX1T1/RUNX1 fusion, CBFß gene rearrangement, PML/RARA fusion, RPN1/MECOM rearrangement, or MLL gene rearrangement is diagnostic for their respective WHO AML classifications.
Acute myeloid leukemia (AML) is a hematopoietic neoplasm characterized by excessive accumulation of myeloid blasts (>20%) in bone marrow, peripheral blood, and other tissues. The identification of specific cytogenetic abnormalities is diagnostic for specific AML subtypes and can be powerful predictors of prognosis and response to therapy. Overall, cytogenetic abnormalities are identified in approximately 55% of adults at diagnosis, with a range of 50-80%; however, only a subset of these chromosome changes is associated with clinical, morphological, and immunophenotypic specificity for a particular AML subtype. These are included in the WHO classification scheme under AML with recurrent genetic abnormalities and include: AML with t(8;21)(q22;q22); AML with inv(3)(q21q26.2) or t(3;3)(q21;q26.2); (RPN1/EVI1 [MECOM]); RUNX1T1/RUNX1, AML with inv(16)(p13q22) or t(16;16)(p13;q22);(CBFß/MYH11), acute promyelocytic leukemia [AML with t(15;17)(q22;q12); (PML/RARA) and variants], and AML with 11q23 (MLL) abnormalities. BCR/ABL1 gene rearrangement is evaluated if the AML panel is normal.
The following FISH probes detect myeloid-associated abnormalities and are available:
- RUNX1T1/RUNX1 fusion for t(8;21)
- RPN1/EVI1 (MECOM) fusion with inv(3)(q21q26.2) or t(3;3)(q21;q26.2)
- CBFß/MYH11 gene for inv(16)/t(16;16)
- MLL gene rearrangement for t(11q23;var)
- PML/RARA for t(15;17)
- BCR/ABL1 for t(9;22
88271x8, 88275x5, 100-300 cells.
Please contact the Cytogenetics Laboratory if assistance with ordering in EPIC or SOFT is needed.