Lab Test

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Scleroderma (SCL-70) Antibody (IgG)

Scleroderma antibodies, Scleroderma Antibody (SCL-70)

Test Codes

EPIC: LAB6988, Beaker: SCL7A, Antrim #30080

Department

Special Chemistry

Specimen Collection Criteria

Collect: One Gold-top SST tube. (Minimum Whole Blood: 2.0 mL)

Physician Office/Draw Specimen Preparation

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells. Refrigerate (2-8°C or 36-46°F) the centrifuged collection tube within two hours of collection. (Minimum Serum: 0.5 mL)

Preparation for Courier Transport

Transport: Centrifuged collection tube, refrigerated (2-8 °C or 36-46 °F). (Minimum Serum: 0.5 mL)

Rejection Criteria

  • Plasma specimens. 
  • Severely hemolyzed, lipemic or icteric specimens. 

In-Lab Processing

Let specimen clot 30-60 minutes then immediately centrifuge to separate serum from cells. Room temperature is acceptable for a maximum of two hours. (Minimum Serum: 0.5 mL)

Storage

Specimen Stability for Testing:

Centrifuged SST Tubes and Microtainers® with Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): Unacceptable

Red-top Tubes and Microtainers® without Separator Gels
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Serum Specimens (Pour-Overs)
Room Temperature (20-26°C or 68-78.8°F): 2 hours
Refrigerated (2-8°C or 36-46°F): 7 days
Frozen (-20°C/-4°F or below): 2 months

Specimen Storage in Department Prior to Disposal:

Refrigerated (2-8°C or 36-46°F): 7 days

Laboratory

Royal Oak Special Testing Laboratory

Performed

Monday – Friday.
Results available within 2 business days.

Reference Range

Negative:  < 1.0 U
Equivocal:  NA
Positive:  ≥ 1.0 U

Test Methodology

Multiplex Flow Assay.

Interpretation

SCL-70 antibody is seen in 20-60% of scleroderma cases. The presence of SCL-70 antibodies is considered diagnostic for scleroderma if it is the only ENA antibody present. Scl-70 is also seen in approximately 25% of progressive systemic sclerosis (PSS) cases.

Clinical Utility

SCL-70 antibody assay aids in the diagnosis of scleroderma and progressive systemic sclerosis (PSS).

CPT Codes

86235

Contacts

Last Updated

2/10/2024

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