Lab Test

Pyruvate Kinase

Test Codes

ARUP #80290, EPIC: LAB6143, SOFT: XPK

Specimen Collection Criteria

Collect (preferred specimen): One Lavender-top EDTA tube.
Also acceptable: One Yellow-top ACD tube or Dark Green-top Heparin tube.

Critical refrigerated. Refrigerate the specimen immediately after collection. 

Physician Office/Draw Specimen Preparation

Do not centrifuge or freeze. Refrigerate (2-8°C or 36-46°F) specimen immediately and maintain refrigerated prior to transport.

Preparation for Courier Transport

Transport: 1.0 mL whole blood, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL)

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Do not centrifuge or freeze. Refrigerate (2-8°C or 36-46°F) specimen immediately and maintain refrigerated.

Transport: 1.0 mL whole blood, refrigerated (2-8°C or 36-46°F). (Minimum: 0.5 mL)


Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): 20 days
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.


Sent to ARUP Laboratories, Salt Lake City, UT.


Sunday – Saturday.
Results available in 2-4 days.

Reference Range

4.6 – 11.2 U/g Hgb.

Test Methodology

Quantitative Enzymatic.

Clinical Utility

Congenital non-spherocytic hemolytic anemia is commonly caused by a deficiency of pyruvate kinase (PK). PK is a glycolytic enzyme. PK enzyme is not sufficient in the RBC's in congenital non-spherocytic hemolytic anemia. The clinical expression of this anemia varies in its severity and requires that both chromosomes carry the defect. The erythrocytes of carriers contain about half the normal PK activity. The diagnostic value of increased PK levels has not been determined. Elevated RBC PK levels have been seen in many disease states associated with anemias.

CPT Codes



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