Lab Test

Prekallikrein Assay

Prekallikrein (Fletcher Factor) Assay

Test Codes

LabCorp #500194, Esoterix #300121, EPIC: LAB6321, SOFT: XPREK

Specimen Collection Criteria

Collect: Two Light Blue-top Sodium Citrate tubes.

TUBES MUST BE FULL.

Physician Office/Draw Specimen Preparation

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 4 hours of collection. If transport within 4 hours is not possible, the following procedure must be followed for accurate results:

  1. Centrifuge the capped tubes at 1500 x g for 15 minutes.
  2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
  3. Immediately remove only the top two-thirds of the PPP and transfer into TWO plastic aliquot tubes.
  4. Freeze (-20°C/-4°F or below) the PPP aliquots immediately.
  5. Transport frozen on DRY ICE. Specimen must remain frozen during transport.

Preparation for Courier Transport

Transport: TWO 1.0 mL PPP aliquots, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL each aliquot)

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

For optimal results, maintain whole blood specimen at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport whole blood to the Laboratory within 4 hours of collection. If transport within 4 hours is not possible, the following procedure must be followed for accurate results:

  1. Centrifuge the capped tubes at 1500 x g for 15 minutes.
  2. Transfer plasma with plastic pipette into a plastic polypropylene centrifuge tube, cap and centrifuge an additional 15 minutes at 1500 x g to obtain platelet poor plasma (PPP) which has a platelet count less than 10 bil/L. Plasma with a platelet count of less than 10 bil/L is critical for accurate results.
  3. Immediately remove only the top two-thirds of the PPP and transfer into TWO plastic aliquot tubes.
  4. Freeze (-20°C/-4°F or below) the PPP aliquots immediately.
  5. Transport frozen on DRY ICE. Specimen must remain frozen during transport.

Transport: TWO 1.0 mL PPP aliquots, frozen (-20°C/-4°F or below). (Minimum: 0.5 mL each aliquot)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 4 hours
Refrigerated (2-8°C or 36-46°F): 4 hours
Frozen (-20°C/-4°F or below): 90 days

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to LabCorp Esoterix Endocrinology, Calabassas Hills, CA.

Performed

Monday.
Results available in 3-8 days.

Reference Range

By Report.

Test Methodology

Clotting Time Assay.

CPT Codes

85292

Contacts

Last Updated

4/1/2020

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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.