Lab Test

Pregnenolone

Test Codes

ARUP #92334, EPIC: LAB6135, SOFT: XPRGN

Instructions

A number of drugs are known to influence endocrine function directly, or to interfere with assay methodology. Whenever possible, physicians should consider discontinuing all drugs, except those necessary to the patient's well-being, several days prior to sample collection. Always note any drugs being administered on the Test Request Form.

Specimen Collection Criteria

Collect (preferred specimen): One Gold-top SST tube.
Also acceptable: One plain Red-top tube or Dark Green Lithium or Sodium Heparin tube or Lavender EDTA.

Physician Office/Draw Specimen Preparation

Critical frozen. Maintain frozen (-20°C/-4°F or below) prior to transport.  Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within two hours of collection. Transfer serum to a plastic transport tube and freeze (-20°C/-4°F or below) immediately.

Preparation for Courier Transport

Transport: 0.5 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.25 mL)

Rejection Criteria

Specimens not collected and processed as indicated.

In-Lab Processing

Critical frozen.

Let specimen clot 30-60 minutes then centrifuge to separate serum from cells within one hour of collection. Transfer serum to a plastic transport tube and freeze (-20°C/-4°F or below) immediately.

Transport: 0.5 mL serum, frozen (-20°C/-4°F or below). (Minimum: 0.25 mL)

Storage

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): Unacceptable
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): 6 months

Specimen Storage in Department Prior to Disposal:

Specimen retention time is determined by the policy of the reference laboratory. Contact the Send Outs Laboratory with any questions.

Laboratory

Sent to ARUP Laboratories, Salt Lake City, UT.

Performed

Monday – Friday.
Results are available in 2-5 days.

Reference Range

By report.

Test Methodology

Quantitative High Performance Liquid Chromatography/Tandem Mass Spectrometry (HPLC-MS/MS).

CPT Codes

84140

Contacts

Last Updated

4/1/2020

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This directory currently reflects information only for specimens collected and/or processed at the
Farmington Hills, Grosse Pointe, Royal Oak, and Troy campuses.